Results 101 to 110 of about 1,658 (208)
Relative FPKMs of innate immune-related genes in silkworm after BmNPV infection in different resistant strains.
Dong-Qiong Fei (2742565) +7 more
core +1 more source
Bmapaf-1 is Involved in the Response against BmNPV Infection by the Mitochondrial Apoptosis Pathway
Discovery of the anti-BmNPV (Bombyx mori nuclearpolyhedrovirus) silkworm strain suggests that some kind of antiviral molecular mechanism does exist but is still unclear.
Qian-ying Chen +7 more
core +1 more source
Bombyx mori nucleopolyhedrovirus (BmNPV) is the most serious viral disease in silkworms. To investigate the mechanisms of the immune responses of B. mori to a BmNPV infection, a suitable reference gene (RG) is necessary for normalizing data when studying
Z Nie +7 more
doaj
BmSpry inhibited BmNPV replication in cultured cells.
(A) BmE cells pretreated with dsRNA as indicated, the dsRNA of dsRed was used as a negative control. At 3 days post transfection, total RNA was extracted and qPCR was used to analyze the BmSpry expression level.
Liang Jiang (146314) +7 more
core +1 more source
Establishment of a novel Method for determination of Bculovirus titer and BmNPV/Silkworm expression system [PDF]
BmNPV/家蚕表达系统是一个以杆状病毒BmNPV为外源基因载体,以家蚕为受体的真核表达系统,具有外源蛋白表达量高、表达产物能进行折叠和修饰具有生物活性、安全、高效等特点。家蚕作为该系统的表达载体,是目前唯一可大规模人工饲养的经济昆虫,且饲养成本极为低廉,该系统在基因工程药物、疫苗等方面具有广阔的应用前景。为了在BmNPV/家蚕表达系统中最大程度、稳定地表达重组蛋白,有必要准确测定病毒滴度。目前已报道多种滴度鉴定方法,但大都存在耗时长、操作繁琐、稳定性不高等缺点,且大部分方法仅适用于AcMNPV ...
马珂
core
A Multiplex CRISPR/Cas9 System for Use as an Anti-BmNPV Therapeutic
Clustered regularly interspaced short palindromic repeats/associated protein 9 nuclease (CRISPR/Cas9) technology guided by a single-guide RNA (sgRNA) has recently opened a new avenue for antiviral therapy. A unique capability of the CRISPR/Cas9 system is multiple genome engineering. However, there are few applications in insect viruses by a single Cas9
Zhanqi Dong +8 more
openaire +2 more sources
Data_Sheet_1_Knockdown of BmTCP-1β Delays BmNPV Infection in vitro.pdf
The molecular mechanism of silkworm resistance to Bombyx mori nucleopolyhedrovirus (BmNPV) infection remains unclear. The chaperonin containing t-complex polypeptide 1 (TCP-1) is essential for the folding of tubulin and actin to produce stable and ...
Mu-wang Li (6497561) +7 more
core +1 more source
The expression pattern of BmTHYs in BmN cells infected with BmNPV.
The BmN cells were infected with BmNPV and samples were collected at different hours to examine the expression of BmTHYs. The mass of each lane’s total protein were 30 μg, and the sample were equalized.
Peng Lü (5655136) +6 more
core +1 more source
Discovery and functional analysis of a new gene (Bm123) in silkworm (Bombyx mori)
Previously our research group used the microarray analysis and suppression subtractive hybridization technologies to find a Bombyx mori resistance related gene (NCBI ID: NP_001153678.1) to B. mori nucleopolyhedrovirus (BmNPV) and the gene was named Bm123.
L Sun +6 more
doaj

