Results 1 to 10 of about 364,753 (312)

Gene editing of SAMHD1 in macrophage-like cells reveals complex relationships between SAMHD1 phospho-regulation, HIV-1 restriction, and cellular dNTP levels

mBio, 2023
Sterile α motif and HD domain-containing protein 1 (SAMHD1) is a dNTP triphosphate triphosphohydrolase (dNTPase) and a potent restriction factor for immunodeficiency virus 1 (HIV-1), active in myeloid and resting CD4+ T cells.
Moritz Schüssler, Kerstin Schott, Nina Verena Fuchs, Adrian Oo, Morssal Zahadi, Paula Rauch, Baek Kim, Renate König   +7 more
doaj   +1 more source

Visualizing Pyrazinamide Action by Live Single-Cell Imaging of Phagosome Acidification and Mycobacterium tuberculosis pH Homeostasis

mBio, 2022
Mycobacterium tuberculosis segregates within multiple subcellular niches with different biochemical and biophysical properties that, upon treatment, may impact antibiotic distribution, accumulation, and efficacy.
Pierre Santucci, Beren Aylan, Laure Botella, Elliott M. Bernard, Claudio Bussi, Enrica Pellegrino, Natalia Athanasiadi, Maximiliano G. Gutierrez   +7 more
doaj   +1 more source

Pseudomonas aeruginosa OprF plays a role in resistance to macrophage clearance during acute infection

Scientific Reports, 2021
While considered an extracellular pathogen, Pseudomonas aeruginosa has been reported to be engulfed by macrophages in cellular and animal models. However, the role of macrophages in P. aeruginosa clearance in vivo remains poorly studied.
Malika Moussouni, Laurence Berry, Tamara Sipka, Mai Nguyen-Chi, Anne-Béatrice Blanc-Potard   +4 more
doaj   +1 more source

Succession in the caecal microbiota of developing broilers colonised by extended-spectrum β-lactamase-producing Escherichia coli

Animal Microbiome, 2022
Background Broilers are among the most common and dense poultry production systems, where antimicrobials have been used extensively to promote animal health and performance.
Ingrid Cárdenas-Rey, Teresita d. J. Bello Gonzalez, Jeanet van der Goot, Daniela Ceccarelli, Gerwin Bouwhuis, Danielle Schillemans, Stephanie D. Jurburg, Kees T. Veldman, J. Arjan G. M. de Visser, Michael S. M. Brouwer   +9 more
doaj   +1 more source

High content quantitative imaging of Mycobacterium tuberculosis responses to acidic microenvironments within human macrophages

FEBS Open Bio, 2023
Intracellular pathogens such as Mycobacterium tuberculosis (Mtb) have evolved diverse strategies to counteract macrophage defence mechanisms including phagolysosomal biogenesis.
Beren Aylan, Laure Botella, Maximiliano G. Gutierrez, Pierre Santucci   +3 more
doaj   +1 more source

Intracellular localisation of Mycobacterium tuberculosis affects efficacy of the antibiotic pyrazinamide

Nature Communications, 2021
Effective chemotherapies against tuberculosis must kill the pathogen’s intracellular population. Here, Santucci et al. show that bacterial localisation within human macrophages impact pyrazinamide (PZA) accumulation and efficacy, and that combination ...
Pierre Santucci, Daniel J. Greenwood, Antony Fearns, Kai Chen, Haibo Jiang, Maximiliano G. Gutierrez   +5 more
doaj   +1 more source

Genotypic virulence profiles and associations in Salmonella isolated from meat samples in wet markets and abattoirs of Metro Manila, Philippines

BMC Microbiology, 2022
Background Salmonella are pathogenic foodborne bacteria with complex pathogenicity from numerous virulence genes housed in Salmonella pathogenicity islands (SPIs), plasmids, and other gene cassettes.
Rance Derrick N. Pavon, Paolo D. G. Mendoza, Camille Andrea R. Flores, Alyzza Marie B. Calayag, Windell L. Rivera   +4 more
doaj   +1 more source

Induced pluripotent stem cell line (PEIi003-A) derived from an apparently healthy male individual

Stem Cell Research, 2020
Induced pluripotent stem cells (iPSCs) are a useful tool to investigate pathomechanistic and cellular processes due to their differentiation potential into different somatic cell types in vitro.
Nina V. Fuchs, Maximilian Schieck, Michaela Neuenkirch, Christiane Tondera, Heike Schmitz, Doris Steinemann, Gudrun Göhring, Renate König   +7 more
doaj   +1 more source

A brain cyst load-associated antigen is a Toxoplasma gondii biomarker for serodetection of persistent parasites and chronic infection

BMC Biology, 2021
Background Biomarker discovery remains a major challenge for predictive medicine, in particular, in the context of chronic diseases. This is true for the widespread protozoan Toxoplasma gondii which establishes long-lasting parasitism in metazoans ...
Céline Dard, Christopher Swale, Marie-Pierre Brenier-Pinchart, Dayana C. Farhat, Valeria Bellini, Marie Gladys Robert, Dominique Cannella, Hervé Pelloux, Isabelle Tardieux, Mohamed-Ali Hakimi   +9 more
doaj   +1 more source

Acute Inflammation Confers Enhanced Protection against Mycobacterium tuberculosis Infection in Mice

Microbiology Spectrum, 2021
Inflammation plays a crucial role in the control of Mycobacterium tuberculosis infection. In this study, we demonstrate that an inflammatory pulmonary environment at the time of infection mediated by lipopolysaccharide treatment in mice confers enhanced ...
Tucker J. Piergallini, Julia M. Scordo, Paula A. Pino, Larry S. Schlesinger, Jordi B. Torrelles, Joanne Turner   +5 more
doaj   +1 more source