A new Bombyx mori larval ovarian cell line highly susceptible to nucleopolyhedrovirus [PDF]
, 2006 Lepidopteran cell lines constitute the backbone for studying baculoviral biology in culturo and for baculovirus vector based recombinant protein expression systems. In the present study, we report establishment of a new continuous cell line designated as
Kanginakudru, Sriramana +5 more
core +1 more source
Research on expression of HIV-1 env in baculovirus [PDF]
, 2000 昆虫细胞杆状病毒表达系统(BEVS)是真核表达系统的一种，以一种无需额外辅助的重组昆虫杆状病毒作为表达载体，目前已被应用来表达不同来源的基因，如细菌、病毒、植物和哺乳动物的基因。它具有表达产量高，蛋白经过完善的后处理过程，如内含子剪切、糖基化等特点。其原理是通过外源基因置换病毒的核多角体蛋白基因，使外源基因在核多角体蛋白启动子下高效表达。本论文中采用BEVS表达HIV-1包膜蛋白，目的在于弥补原核表达系统不能表达大片段的、高度糖基化的重组蛋白；并探索其应用在检测试剂中的前景。Baculovirus ...
郭庆
core
Short hairpin RNA expression for enhancing the resistance of Bombyx mori (Bm) to nucleopolyhedrovirus in vitro and in vivo [PDF]
, 2016 A new paradigm of RNAi technology has been studied for enhancing the resistance to virus in plants and animals. Previous studies have shown that the Bombyx mori (Bm) U6 promoter based shRNA is an effective tool for inducing RNAi in Bombyx mori cell line.
Bhaskar, Roy +5 more
core +2 more sources
High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization [PDF]
, 2009 Background Budded baculoviruses are utilized for vaccine, the production of antibody and functional analysis of transmembrane proteins. In this study, we tried to produce and purify the recombinant Bombyx mori nucleopolyhedrovirus (rBmNPV-hPRR) that ...
Kato, Tatsuya +3 more
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Subcellular localization of Bombyx mori ribosomal protein S3a and effect of its over-expression on BmNPV infection [PDF]
, 2012 In the present study, using a BV/PH-Bms3a-EGFP, we found that Bombyx mori ribosomal protein S3a (BmS3a) with EGFP fused to its C-terminal, was predominantly localized in the cytoplasm of B. mori cells.
Hui-ling, W +6 more
core +2 more sources
Geração e acumulo de mutantes com poucos poliedros após passagem serial do baculovirus anticarsia (AgMNPV) em cultura de células. [PDF]
, 2016 bitstream/CENARGEN/29412/1/bp183 ...
CASTRO, M. E. B. de +2 more
core +1 more source
Silkworm expression system as a platform technology in life science [PDF]
, 2009 Many recombinant proteins have been successfully produced in silkworm larvae or pupae and used for academic and industrial purposes. Several recombinant proteins produced by silkworms have already been commercialized.
A Miyajima +77 more
core +2 more sources
Thermostability of the Recombinant Haemagglutinin-Neuraminidase Glycoproteins of Newcastle Disease Virus [PDF]
, 2003 The haemagglutinin-neuraminidase (HN) glycoprotein of Newcastle disease virus (NDV) is of primary importance in inducing virus-neutralizing antibodies against viral infection in chicken and has been used in the development of many vaccines.
Tang, Yik Kiong
core
Insects, 2020 Previous studies have revealed that some proteins in Bombyx mori larvae digestive juice show antiviral activity. Here, based on the label-free proteomics data, BmLipase member H-A (BmLHA) was identified as being involved in the response to BmNPV ...
Shang-Zhi Zhang +9 more
doaj +1 more source
The Baculovirus Uses a Captured Host Phosphatase to Induce Enhanced Locomotory Activity in Host Caterpillars [PDF]
, 2012 The baculovirus is a classic example of a parasite that alters the behavior or physiology of its host so that progeny transmission is maximized. Baculoviruses do this by inducing enhanced locomotory activity (ELA) that causes the host caterpillars to ...
BA Keddie +43 more
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