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Phenotypic grouping of 141 BmNPVs lacking viral gene sequences

open access: yesPhenotypic grouping of 141 BmNPVs lacking viral gene sequences
We constructed a series of gene knockout BmNPVs (KOVs) for each of 141 genes using the BmNPV T3 bacmid system and lambda red recombination system. In a subsequent analysis of the properties needed for infection using a marker gene, egfp (enhanced green fluorescent protein gene), inserted into the polyhedrin locus, the knockout viruses (KOVs) were ...
openaire  

Engineering back-splicing-like sites for enhanced linear RNA expression. [PDF]

open access: yesAppl Microbiol Biotechnol
Chen X   +9 more
europepmc   +1 more source

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