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Bombyx mori Rad23 (BmRad23) contributes to the repair of UV-damaged BmNPV

Pesticide Biochemistry and Physiology, 2020
Baculoviruses have been developed as long-term and environmentally friendly biopesticides. However, solar ultraviolet radiation can reduce the activity of baculovirus. Radiation sensitive 23 (Rad23) can recognize DNA damage and is involved with nucleotide excision repair (NER).
Qi, Tang   +8 more
openaire   +2 more sources

Use of RNAi technology to confer enhanced resistance to BmNPV on transgenic silkworms

Archives of Virology, 2003
dsRNA is a powerful tool for gene-specific silencing in plants and animals. In this study, we examined the use of gene silencing in generating transgenic silkworms resistant to the Bombyx mori nucleopolyhedrovirus (BmNPV). Using a transposon piggyBac system, we first generated BmN cells (rBmN-lef1), which carried artificial genes designed for ...
R, Isobe   +8 more
openaire   +2 more sources

[The identification of BmNPV gp64 envelope gene].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2004
gp64 glycoprotein, an abundant and the only membrane glycoprotein encoded by the baculovirus genome, contains a signal peptide sequence at N-terminus, a transmembrane anchor at c-terminus and a ectodomain between them. The efficient signal sequences and the transmembrane anchor sequences of AcMNPV gp64 gene have been exploited in baculovirus expression
Y, Wang   +4 more
openaire   +1 more source

Acetylation of DnaJ facilitates the proliferation of BmNPV by affecting the transport of nucleocapsids

Microbial Pathogenesis
DnaJ (Orf40), a late-expressed factor of Bombyx mori nucleopolyhedrovirus (BmNPV), is essential for the budding of virions and influences the transfer of the nucleocapsid from the nucleus to the cytoplasm. Previous studies showed that the knockdown of dnaj could prevent the nucleocapsid from exiting the nucleus, but the underlying regulatory mechanism ...
Jiaqi Li   +6 more
openaire   +2 more sources

สัณฐานวิทยาของผลึกโปรตีนไวรัส BmNPV จากหนอนไหมในประเทศไทย

1, 8, วารสารวิจัย มสด สาขาวิทยาศาสตร์และเทคโนโลยี
openaire   +1 more source

In vivo RNA interference of BmNHR96 enhances the resistance of transgenic silkworm to BmNPV

Biochemical and Biophysical Research Communications, 2017
We previously identified a nuclear hormone receptor gene, BmNHR96, which promotes Bombyx mori nucleopolyhedrovirus (BmNPV) entry into silkworm cells. In an attempt to create an antiviral silkworm strain for better silk production, we used RNAi to downregulate BmNHR96 in silkworm larvae.
Ji-Gui, Yang   +8 more
openaire   +2 more sources

BmWARS inhibits BmNPV infection via the PI3K-Akt pathway

Bulletin of Entomological Research
AbstractBombyx mori Tryptophanyl-tRNA synthetase (BmWARS) belongs to the family of Ic-like aminoacyl-tRNA synthetases (aaRSs), whose specific recognition of the substrate Trp, tRNA, maintains the fidelity of protein synthesis. In this study, BmWARS was cloned and characterized from the midgut of the silkworm, Bombyx mori, resulting in an open reading ...
Jinyang Wang   +10 more
openaire   +2 more sources

Fast identification of the BmNPV infected silkworms by portable NIR spectroscopy and chemometrics

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
A convenient, low-cost, and rapid detection of BmNPV-infected silkworms is of great significance for the safety of the sericulture industry. In this study, a portable NIR system was used to collect the spectra of normal silkworms and the infected silkworms induced by the administration of Bombyx mori nuclear polyhedrosis virus (BmNPV).
Yihan Liu   +5 more
openaire   +2 more sources

The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue

In Vitro Cellular & Developmental Biology - Animal, 2008
The effect of Bombyx mori nuclear polyhedrosis virus (BmNPV) on biochemical changes of TC-100 medium containing 10% fetal bovine serum (FBS) in embryonic primary cultures of silkworm was investigated. The primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums (diluted with TC-100 medium representing multiplicity ...
Matindoost, Leila   +4 more
openaire   +5 more sources

[Mechanism analysis of Anti-BmNPV resistant strain NC99R].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2020
Bombyx mori is a lepidopteran insect with important economic value. Bombyx mori nucleopolyhedrovirus (BmNPV) causes huge economic loss to silkworm industry in China every year. The objective of this study is to determine the anti-BmNPV mechanism of Bombyx mori strain NC99R, and to provide a basis for understanding the molecular mechanism of the ...
Zhanqi, Dong   +6 more
openaire   +1 more source

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