Results 131 to 140 of about 1,113 (177)

The piRNA response to BmNPV infection in the silkworm fat body and midgut

Insect Science, 2020
AbstractBombyx mori nucleopolyhedrovirus (BmNPV) is a DNA virus that causes huge losses to the silkworm industry but the piRNA responses during BmNPV infection in the silkworm remain uninvestigated. Here, silkworm piRNA profiles of uninfected and BmNPV‐infected fat body and midgut were determined by high‐through sequencing in the early stages of BmNPV ...
Min Feng, Anna Kolliopoulou, Yao‐Hong Zhou, Shi‐Gang Fei, Jun‐Ming Xia, Luc Swevers, Jing‐Chen Sun   +6 more
openaire   +2 more sources

BmNPV circular RNA-encoded peptide VSP39 promotes viral replication

International Journal of Biological Macromolecules, 2023
CircRNAs are covalently closed single-stranded circular RNA molecules, which are not easily degraded by endonucleases and play vital roles in many biological processes. Currently, most studies on circRNAs focus on endogenous circRNAs in cells, and there are few studies on virus-encoded circRNAs.
Yaxin, Zhang, Xing, Zhang, Zeen, Shen, Qunnan, Qiu, Xinyu, Tong, Jun, Pan, Min, Zhu, Xiaolong, Hu, Chengliang, Gong   +8 more
openaire   +2 more sources

Comparative analysis of the intestinal flora of BmNPV-resistant and BmNPV-sensitive silkworm varieties

Microbial Pathogenesis
Bombyx mori nucleopolyhedrovirus (BmNPV) is a very common and infectious virus that affects silkworms and hinders silk production. To investigate the intestinal flora of BmNPV-resistant and BmNPV-sensitive silkworm varieties, 16 S rDNA high-throughput sequencing was performed.
Xu, Yang, Pai, Liu, Haodong, Yu, Min, Ling, Mingzhen, Ma, Qiang, Wang, Xudong, Tang, Zhongyuan, Shen, Yiling, Zhang   +8 more
openaire   +2 more sources

Over expression of bmo-miR-2819 suppresses BmNPV replication by regulating the BmNPV ie-1 gene in Bombyx mori

Molecular Immunology, 2019
Bombyx mori nucleopolyhedrovirus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Accumulating studies in recent years suggest that insect viruses infection can change the host microRNAs (miRNAs) expression profile and both cellular and viral miRNAs play roles in host-pathogen interactions. Until now,
Ping, Wu, Qi, Shang, Owusu Amanfo, Dweteh, Haoling, Huang, Shaolun, Zhang, Jinbo, Zhong, Qirui, Hou, Xijie, Guo   +7 more
openaire   +2 more sources

Bombyx mori Tetraspanin A (BmTsp.A) is a facilitator in BmNPV invasion by regulating apoptosis

Developmental & Comparative Immunology, 2023
BmTsp.A (Bombyx mori Tetraspanin A) is one of the four transmembrane proteins which are capable to regulate multiple aspects of the immune response and are involved in various stages of viral invasion of the hosts. This study focused on the sequence features, analysis of expression pattern, as well as the effect of BmTsp.A on BmNPV (Bombyx mori ...
Xianghan Mei, Peitong Qiao, Hengheng Ma, Siyu Qin, Xia Song, Qiaoling Zhao, Dongxu Shen   +6 more
openaire   +2 more sources

Isolation of ferritin and its interaction with BmNPV in the silkworm, Bombyx mori

Developmental & Comparative Immunology, 2018
Ferritin is a ubiquitous iron storage protein that plays an important role in host defence against pathogen infections. In the present study, native ferritin was isolated from the hemolymph of Bombyx mori using native-polyacrylamide gel electrophoresis (native-PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Dong-Qiong, Fei, Hai-Zhong, Yu, Jia-Ping, Xu, Shang-Zhi, Zhang, Jie, Wang, Bing, Li, Li-Ang, Yang, Pei, Hu, Xin, Xu, Kang, Zhao, Toufeeq, Shahzad   +10 more
openaire   +2 more sources

Identification of key metabolic pathways reprogrammed by BmNPV in silkworm Bombyx mori

Journal of Invertebrate Pathology, 2022
Elucidating the mechanism of infection of Bombyx mori nuclear polyhedrosis virus (BmNPV) and host antiviral response remains a major scientific task in sericulture. Virus invasion causes a series of antiviral immune responses in the host, and successful infection leads to massive changes in the host's physiological and biochemical state.
Weiwei, Zhou, Dandan, Song, Han, Chen, Qi, Tang, Qian, Yu, Shuhao, Huo, Xiaoyong, Liu, Keping, Chen, Feifei, Zhu   +8 more
openaire   +2 more sources

CRISPR/Cpf1 multiplex genome editing system increases silkworm tolerance to BmNPV

International Journal of Biological Macromolecules, 2022
The CRISPR/Cas9 genome editing technology is now widely used in insect studies, but the use of CRISPR can be further increased to improve insect genome engineering. We established a direct mutation at multiple loci in several genes simultaneously used by CRISPR/Cpf1 multiplex genome editing technology to target the BmNPV genome.
Xuan, Pan, Yan, Luo, Nachuan, Liao, Ya, Zhang, Miao, Xiao, Peng, Chen, Cheng, Lu, Zhanqi, Dong   +7 more
openaire   +2 more sources

Comprehensive transcriptome sequencing of silkworm Midguts: Uncovering extensive isoform diversity and alternative splicing in BmNPV-Sensitive and BmNPV-resistant strains

Journal of Invertebrate Pathology
The silkworm, Bombyx mori, stands out as one of the few economically valuable insects within the realm of model organisms. However, Bombyx mori nucleopolyhedrovirus (BmNPV) poses a significant threat, decreasing the quality and quantity of silkworm cocoons.
Min Tang, Yi Liu, Hantao Zhang, Lindan Sun, Peng Lü, Keping Chen   +5 more
openaire   +2 more sources

Production of scFv‐displaying BmNPV in silkworm larvae and its efficient purification

Biotechnology and Applied Biochemistry, 2010
Baculovirus‐display technology utilizing the gp64 envelope protein has been developed. A simple and efficient process to separate the virus from the majority of the protein contaminants may be needed for the future demand of pure and functional baculovirus vectors ideal for vaccine‐ and gene‐delivery applications.
Takashi, Dojima, Takuya, Nishina, Tatsuya, Kato, Hiroshi, Ueda, Enoch Y, Park   +4 more
openaire   +2 more sources