Results 131 to 140 of about 679 (168)

Identification of key metabolic pathways reprogrammed by BmNPV in silkworm Bombyx mori

Journal of Invertebrate Pathology, 2022
Elucidating the mechanism of infection of Bombyx mori nuclear polyhedrosis virus (BmNPV) and host antiviral response remains a major scientific task in sericulture. Virus invasion causes a series of antiviral immune responses in the host, and successful infection leads to massive changes in the host's physiological and biochemical state.
Weiwei, Zhou, Dandan, Song, Han, Chen, Qi, Tang, Qian, Yu, Shuhao, Huo, Xiaoyong, Liu, Keping, Chen, Feifei, Zhu   +8 more
openaire   +2 more sources

Bombyx mori Tetraspanin A (BmTsp.A) is a facilitator in BmNPV invasion by regulating apoptosis

Developmental & Comparative Immunology, 2023
BmTsp.A (Bombyx mori Tetraspanin A) is one of the four transmembrane proteins which are capable to regulate multiple aspects of the immune response and are involved in various stages of viral invasion of the hosts. This study focused on the sequence features, analysis of expression pattern, as well as the effect of BmTsp.A on BmNPV (Bombyx mori ...
Xianghan Mei, Peitong Qiao, Hengheng Ma, Siyu Qin, Xia Song, Qiaoling Zhao, Dongxu Shen   +6 more
openaire   +2 more sources

Isolation of ferritin and its interaction with BmNPV in the silkworm, Bombyx mori

Developmental & Comparative Immunology, 2018
Ferritin is a ubiquitous iron storage protein that plays an important role in host defence against pathogen infections. In the present study, native ferritin was isolated from the hemolymph of Bombyx mori using native-polyacrylamide gel electrophoresis (native-PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Dong-Qiong, Fei, Hai-Zhong, Yu, Jia-Ping, Xu, Shang-Zhi, Zhang, Jie, Wang, Bing, Li, Li-Ang, Yang, Pei, Hu, Xin, Xu, Kang, Zhao, Toufeeq, Shahzad   +10 more
openaire   +2 more sources

CRISPR/Cpf1 multiplex genome editing system increases silkworm tolerance to BmNPV

International Journal of Biological Macromolecules, 2022
The CRISPR/Cas9 genome editing technology is now widely used in insect studies, but the use of CRISPR can be further increased to improve insect genome engineering. We established a direct mutation at multiple loci in several genes simultaneously used by CRISPR/Cpf1 multiplex genome editing technology to target the BmNPV genome.
Xuan, Pan, Yan, Luo, Nachuan, Liao, Ya, Zhang, Miao, Xiao, Peng, Chen, Cheng, Lu, Zhanqi, Dong   +7 more
openaire   +2 more sources

Comprehensive transcriptome sequencing of silkworm Midguts: Uncovering extensive isoform diversity and alternative splicing in BmNPV-Sensitive and BmNPV-resistant strains

Journal of Invertebrate Pathology
The silkworm, Bombyx mori, stands out as one of the few economically valuable insects within the realm of model organisms. However, Bombyx mori nucleopolyhedrovirus (BmNPV) poses a significant threat, decreasing the quality and quantity of silkworm cocoons.
Min Tang, Yi Liu, Hantao Zhang, Lindan Sun, Peng Lü, Keping Chen   +5 more
openaire   +2 more sources

Bombyx mori Rad23 (BmRad23) contributes to the repair of UV-damaged BmNPV

Pesticide Biochemistry and Physiology, 2020
Baculoviruses have been developed as long-term and environmentally friendly biopesticides. However, solar ultraviolet radiation can reduce the activity of baculovirus. Radiation sensitive 23 (Rad23) can recognize DNA damage and is involved with nucleotide excision repair (NER).
Qi, Tang, Fangying, Chen, Xinyu, Qi, Peng, Wu, Huiqin, Chen, Lipeng, Qiu, Zhaoyang, Hu, Keping, Chen, Guohui, Li   +8 more
openaire   +2 more sources

Production of scFv‐displaying BmNPV in silkworm larvae and its efficient purification

Biotechnology and Applied Biochemistry, 2010
Baculovirus‐display technology utilizing the gp64 envelope protein has been developed. A simple and efficient process to separate the virus from the majority of the protein contaminants may be needed for the future demand of pure and functional baculovirus vectors ideal for vaccine‐ and gene‐delivery applications.
Takashi, Dojima, Takuya, Nishina, Tatsuya, Kato, Hiroshi, Ueda, Enoch Y, Park   +4 more
openaire   +2 more sources

Use of RNAi technology to confer enhanced resistance to BmNPV on transgenic silkworms

Archives of Virology, 2003
dsRNA is a powerful tool for gene-specific silencing in plants and animals. In this study, we examined the use of gene silencing in generating transgenic silkworms resistant to the Bombyx mori nucleopolyhedrovirus (BmNPV). Using a transposon piggyBac system, we first generated BmN cells (rBmN-lef1), which carried artificial genes designed for ...
R, Isobe, K, Kojima, T, Matsuyama, G X, Quan, T, Kanda, T, Tamura, K, Sahara, S I, Asano, H, Bando   +8 more
openaire   +2 more sources

[The identification of BmNPV gp64 envelope gene].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2004
gp64 glycoprotein, an abundant and the only membrane glycoprotein encoded by the baculovirus genome, contains a signal peptide sequence at N-terminus, a transmembrane anchor at c-terminus and a ectodomain between them. The efficient signal sequences and the transmembrane anchor sequences of AcMNPV gp64 gene have been exploited in baculovirus expression
Y, Wang, Z, Li, Q, Long, G, Nong, X, Wang   +4 more
openaire   +1 more source

Acetylation of DnaJ facilitates the proliferation of BmNPV by affecting the transport of nucleocapsids

Microbial Pathogenesis
DnaJ (Orf40), a late-expressed factor of Bombyx mori nucleopolyhedrovirus (BmNPV), is essential for the budding of virions and influences the transfer of the nucleocapsid from the nucleus to the cytoplasm. Previous studies showed that the knockdown of dnaj could prevent the nucleocapsid from exiting the nucleus, but the underlying regulatory mechanism ...
Jiaqi Li, Siqi Xu, Chaoguang Gu, Xinyi Fan, Xizhen Zhang, Meng Miao, Wei Yu   +6 more
openaire   +2 more sources