Results 171 to 180 of about 11,914 (206)

Sperm Cryopreservation in Boars

Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term ...
Inmaculada, Parrilla, María A, Gil, Cristina, Cuello, Emilio A, Martinez   +3 more
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Dynamics of heparin-binding proteins on boar sperm

Animal Reproduction Science, 2009
The presence, topology and dynamics of heparin-binding proteins (HBP) on boar sperm were evaluated. HBP distribution was analyzed by subcellular parting, using biotinylated heparin followed by colorimetric detection. HBP were detected as peripherical and integral periacrosomal membrane proteins.
Dora G, Dapino, Juan M, Teijeiro, Marcelo O, Cabada, Patricia E, Marini   +3 more
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Efficiency of three boar sperm enrichment techniques

Reproduction in Domestic Animals, 2021
AbstractThe objective of the study was to investigate the efficiency of three enrichment methods to separate boar spermatozoa. Twenty‐four ejaculates from 12 boars (2 ejaculates/boar) were extended (30 × 106 spermatozoa/mL) in commercial Beltsville Thawing Solution.
Athina Basioura, Georgios Tsousis, Constantin M. Boscos, Ioannis A. Tsakmakidis   +3 more
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Reproductive toxicology of 2,4 dinitrophenol in boar sperm

Toxicology in Vitro, 2016
Abstract The aim of this study was to evaluate the toxic effects of 2,4 dinitrophenol (DNP) on swine spermatozoa; particularly plasma membrane fluidity and integrity, mitochondrial function and motility, including oxidative stress parameters of lipid peroxidation and production of reactive oxygen species, when cooling to 17 °C during 24 to 96 h.
Estela Fernandes Silva, Antonio Sergio Varela, Tainã Figueiredo Cardoso, Francieli Moro Stefanello, Ana Cristina Kalb, Pablo Elías Martínez, Carine Dahl Corcini   +6 more
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Controlled freezing studies on boar sperm cryopreservation

Andrologia, 2009
Boar spermatozoa from different males were frozen at a number of cooling rates using a controlled-rate freezing machine designed to minimise thermal variables involved in the cooling process, to see whether inter-boar sperm cryosurvival may be improved by changing cooling rate. Four cooling rates in the range 3 degrees C min(-1) to 24 degrees C min(-1)
A, Medrano, W V, Holt, P F, Watson
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Validation of the sperm mobility assay in boars and stallions

Theriogenology, 2006
The sperm mobility assay used in the present study measures the rate of sperm penetration in a biologically inert cell-separation solution (Accudenz). When a sample of sperm is overlaid in a cuvette containing Accudenz, sperm penetrate the solution and absorbance of the sample can be measured with a spectrophotometer.
Jorge A, Vizcarra, J Joe, Ford
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The effects of prostaglandin F2α on sperm output in boars

Prostaglandins, 1977
The effect of prostaglandin F2alpha (PGF2alpha) on the sperm output of six boars was investigated in two studies. Although PGF2alpha did not significantly affect sperm numbers in the ejaculate, a significantly longer (P less than 0.05) ejaculation of the sperm rich fraction occurred following injection of PGF2alpha.
P H, Hemsworth, J, Donnelly, J K, Findlay, D B, Galloway   +3 more
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Autophosphorylation of boar sperm membranes.

Biochemistry international, 1986
Boar sperm plasma membranes and the membranes released during an in vitro acrosome-like reaction were capable of autophosphorylation. The purified membranes were incubated in Tyrode's buffer containing [32P]ATP with or without Ca2+ and/or diacylglycerol.
M, Nikolopoulou, D A, Soucek, J C, Vary
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Treatment of boar sperm with nanoparticles for improved fertility

Theriogenology, 2019
Continuous progress in nanoscience has allowed the synthesis of various nanoscale particles, known as nanoparticles or nanomaterials which, by harnessing unique physico-chemical properties, are crucial for multiple bio-applications. Despite the revealed toxicity (nanotoxicity) of nanoparticles in various in vitro and in vivo studies, their careful ...
Jean M, Feugang, Carley E, Rhoads, Popoola A, Mustapha, Steve, Tardif, John J, Parrish, Scott T, Willard, Peter L, Ryan   +6 more
openaire   +2 more sources

Inhibition of glycolysis in boar sperm by α-chlorohydrin

Contraception, 1980
The effect of alpha-chlorohydrin (3-chloropropan-1,2-diol) on the metabolism of D-[U-14C]-fructose and L-[U-14C]-lactate by washed boar sperm has been investigated. Whereas alpha-chlorohydrin at concentrations as low as 0.1 mM inhibited the metabolism of fructose and led to an increase in the utilization of endogenous lactate, amounts up to 500 mM had ...
P, Hutton, A G, Dawson, A R, Jones
openaire   +2 more sources