Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Natural Boundary Element Method for Mixed Problems of Stokes Flow in Exterior Circular Region
:Aiming at the velocity-pressure mixed boundary problems of Stokes flow in exterior circular region, based on the character of the function of complex variable, the natural boundary element theory and series expanded method,the Poisson integral formula ...
Peng Weihong, Dong Zhengzhu, Cao Guohua
doaj
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
The paper investigates a Dirichlet-type boundary value problem for a three-dimensional elliptic equation with three singular coefficients in the first octant.
K.T. Karimov, M.R. Murodova
doaj +1 more source
Shielding properties of a conducting bar calculated with a boundary integral method [PDF]
A plane rectangular bar of conducting and permeable material is placed in an external low-frequency magnetic field. The shielding properties of this object are investigated by solving the given plane eddy current problem for the vector potential with the
L. O. Fichte +3 more
doaj
The Approximate Solution of 2D Dirichlet Problem in Doubly Connected Domains
We propose a new method for constructing an approximate solution of the two-dimensional Laplace equation in an arbitrary doubly connected domain with smooth boundaries for Dirichlet boundary conditions.
Atallah El-shenawy, Elena A. Shirokova
doaj +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source
Reduction of Boundary Value Problem to Possio Integral Equation in Theoretical Aeroelasticity
The present paper is the first in a series of works devoted to the solvability of the Possio singular integral equation. This equation relates the pressure distribution over a typical section of a slender wing in subsonic compressible air flow to the ...
A. V. Balakrishnan, M. A. Shubov
doaj +1 more source

