We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Prescribed Performance Attitude-Tracking Control for Rigid Satellite Under External Disturbance. [PDF]
Zhang C, Wang T, Wan M, Li T.
europepmc +1 more source
Atomic decomposition of anisotropic Herz-Amalgam spaces and boundedness of sublinear operators
Mehvish Sultan +3 more
openalex +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Improved intelligent decision model for electric vehicle battery using q-Rung orthopair fuzzy Schweizer-Sklar prioritized Z-information. [PDF]
Ali A, Shaikh HU, Ashraf S, Tulu A.
europepmc +1 more source
Uniform Stability and Boundedness of a Kind of Third Order Delay Differential Equations
Moussadek Remili, Lynda D. Oudjedi
openalex +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Safe-RTSKF: A Safety-Constrained Recursive Two-Stage Kalman Filter for Robust Sensor State Estimation in Strongly Nonlinear Systems. [PDF]
Lin Y +6 more
europepmc +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Predefined-Time Sliding Mode Control of Robotic Manipulators via Artificial Delay Feedback and Reinforcement Learning. [PDF]
Zhang L, Wang J, Wang J, Lu J, Li P.
europepmc +1 more source

