Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Isolation of Highly Pathogenic Avian Influenza A(H5N1) Virus from Fetal Bovine Serum, United States, 2025. [PDF]
Rebelo AR +10 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Evaluating Alternatives to Fetal Bovine Serum in the Development of Advanced Biomaterial-Based Tumor Models: Overcoming Challenges in Biofabrication. [PDF]
Quansah E, Rivera I, Pedrón-Haba S.
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Proceedings of the Twenty-Third Annual Conference (1990). Bovine "Give Away" and Awards
The AABP again sponsored a "Bovine Give-Away" in the form of ten $50.00 prizes. The event was organized by Dr. Samuel Hutchins 3rd, Exhibits Manager in collaboration with the exhibitors. Dr.
Bovine Practitioners, American Association of
core
Comparison of Nerve Growth Factor with Fetal Bovine Serum for Promoting Closure of Defects in Corneal Epithelial Cell Layers. [PDF]
Kozlowski MR.
europepmc +1 more source
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Alternatives for fetal bovine serum (FBS) and sustainability of milk derivatives in cell culture. [PDF]
Pittino OM +11 more
europepmc +1 more source

