Results 61 to 70 of about 680,154 (292)

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Comparison of pasture and concentrate finishing of Holstein Friesian, Aberdeen Angus × Holstein Friesian and Belgian Blue × Holstein Friesian steers [PDF]

, 2010
peer-reviewedCrossbreeding Holstein Friesian dairy cows with both early and late maturing beef breed bulls is common in Ireland. This study concerned the comparison of spring-born Holstein Friesian (HF), Aberdeen Angus × Holstein Friesian (AA) and ...
Keane, Michael G., Moloney, Aidan P
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Short‐Term Exposure to Culture Media Used in Human ART Shapes Early Calcium Oscillations in ICSI‐Fertilized Mouse Oocytes and Impacts Adult Phenotype

Reproductive Medicine and Biology
Purpose The influence of culture media used during in vitro fertilization (IVF) on offspring phenotype remains controversial. However, specific effects of short exposure time after fertilization remain underexplored.
Bernadette Banrezes, Thierry Sainte‐Beuve, Anne Frambourg, Alice Jouneau   +3 more
doaj   +1 more source

What Do Cattle Prefer in a Tropical Climate: Water Immersion or Artificial Shade? [PDF]

, 2012
Animal performance is affected by high air temperature and it is known that shade reduces the absorption of radiant temperature, and water for immersion facilitates heat loss.
Geraldo, A.C.P.M., Pereira, A.M.F., Titto, C.G., Titto, E.A.L.   +3 more
core  

Retrospective study of more than 9000 feline cutaneous tumours in the UK: 2006–2013 [PDF]

, 2017
The aim of the study was to utilise a large database available from a UK-based, commercial veterinary diagnostic laboratory to ascertain the prevalence of different forms of cutaneous neoplasia within the feline population, and to detect any breed, sex ...
Dobromylskyj, M J, Ho, N T, Smith, K C
core   +2 more sources

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Nuut gedink oor die wese en inhoud van die dienswerk van die diaken

HTS Teologiese Studies/Theological Studies, 2012
The essence and content of a deacon’s service revisited. In many congregations, there is great uncertainty about the precise duty of the deacon within the congregation.
Gert Breed
doaj   +3 more sources