Regulatory network of rice in response to heat stress and its potential application in breeding strategy. [PDF]
Mol Breed, 2023 Ma Z +6 more
europepmc +1 more source
The Breeding Ecology of Homed Puffins Fratercula comiculata in Alaska. [PDF]
, 2001 The Horned Puffin {Fratercula corniculata) is one of three North Pacific puffin species. Horned Puffins almost always nest amongst boulders and in rock crevices.
Harding, Ann Marie Aglionby
core
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Redefining crop breeding strategy for effective use of nitrogen in cropping systems. [PDF]
Commun Biol, 2022 Ciampitti IA +3 more
europepmc +1 more source
Optimal Breeding Strategies for Calving Ease
Journal of Dairy Science, 1994 Calving ease is of economic importance in dairy cattle and should be considered in breeding programs. Economic values of direct and maternal calving ease were derived based on cost-benefit analysis and gene flow methodology. Marginal returns from dystocia reduction in primiparous dams were -$43 per phenotypic standard deviation compared with $300 for ...
openaire +2 more sources
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
FEBS Open Bio, EarlyView.Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
A fast breeding strategy creates fragrance- and anthocyanin-enriched rice lines by marker-free gene-editing and hybridization. [PDF]
Mol Breed, 2023 Shi T +10 more
europepmc +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Deep scoping: a breeding strategy to preserve, reintroduce and exploit genetic variation. [PDF]
Theor Appl Genet, 2021 Vanavermaete D +3 more
europepmc +1 more source