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Biophysical contrast mechanisms of Brillouin microscopy
Multimodal and Nanoscale Optical Microscopy, 2023Brillouin scattering spectroscopy, which for decades has been used in applied physics and material research, has recently gained prominence as a microscopy technique in biological research and clinical medicine for the measurement of mechanical properties of cells, tissues and biomaterials.
Fiore, Antonio, Scarcelli, Giuliano
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Three-dimensional Brillouin confocal microscopy
2007 Conference on Lasers and Electro-Optics (CLEO), 2007We present Brillouin confocal microscopy for non-invasively measuring mechanical properties of material with three-dimensional spatial resolution and high sensitivity.
Giuliano Scarcelli, Seok H. Yun
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Dark-field Brillouin microscopy
Optics Letters, 2017Brillouin microscopy is a non-contact and label-free technique for mapping fundamental micro-mechanical properties in the volume of biological systems. Specular reflections and elastic scattering easily overwhelm the weak Brillouin spectra due to the limited extinction of virtually imaged phased array spectrometers, thereby affecting the image ...
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Noninvasive Imaging: Brillouin Confocal Microscopy
2018In the past decades, there has been increased awareness that mechanical properties of tissues and cells are closely associated with disease physiology and pathology. Recognizing this importance, Brillouin spectroscopy instrumentation, already utilized in physics and material science, has been adopted for cell and tissue biomechanics.
Miloš, Nikolić +3 more
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Brillouin microscopy for ocular biomechanics
Conference on Lasers and Electro-Optics 2010, 2010We present Brillouin confocal microscopy with high throughput and high extinction for non-invasive mechanical measurements of tissue and biomaterials. In particular, we demonstrate biomechanical characterization of crystalline lens and cornea.
Giuliano Scarcelli, Seok H. Yun
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Brillouin microscopy: an emerging tool for mechanobiology
Nature Methods, 2019The role and importance of mechanical properties of cells and tissues in cellular function, development and disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations. Recently, Brillouin microscopy, a type of optical elastography, has emerged as a non-destructive, label- and contact ...
Prevedel R. +3 more
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Coaxial line scanning Brillouin microscopy
Optical Elastography and Tissue Biomechanics XIIAbstractConfocal Brillouin microscopy (CBM) enables high-resolution mechanical imaging but has slow acquisition speeds due to its point-by-point scanning strategy. Line-scanning Brillouin microscopy (LSBM) offers imaging acquisition speed improvements but faces challenges such as beam distortion in biaxial configurations and insufficient extinction ...
Chenjun Shi, Jitao Zhang
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Brillouin Scanning Microscopy in Keratoconus
2016The mechanical properties of the cornea are thought to be central in the development of keratoconus and other corneal ectatic disorders. However, current technologies to measure mechanical properties are limited for in vivo and/or in situ applications. We have recently introduced a new approach, based on optical Brillouin scattering, toward developing ...
Giuliano Scarcelli, Seok Hyun Yun
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Probing Mechanical Properties in Biology Using Brillouin Microscopy
Trends in Cell Biology, 2019Brillouin microscopy can be used to map the mechanical properties of samples in a noncontact and label-free manner, with potential applications in cell biology. Here, we provide an overview of the underlying principles and technology as well as the current challenges and outlook.
Elsayad, Kareem +2 more
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Microwave induced phonons imaging by Brillouin microscopy
IEEE Ultrasonics Symposium, 2005., 2006Bulk Acoustic Waves (BAW) as well as Surface Acoustic Waves (SAW) have been studied by Brillouin Spectroscopy (BS). Experiments performed on Lithium Niobate single crystals and Zinc Oxide thin films have pointed out that BS is able to detect piezoelectrically induced phonons.
B. Vincent +7 more
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