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Brillouin Scattering Microscopy for Mechanical Imaging [PDF]
In a world where science is constantly challenged to solve problems of increasing complexity, light is paving new ways to gather information about the physical properties of matter. Among these properties, elasticity is becoming fundamental in the understanding and the diagnosis of several diseases.
Antonacci, Giuseppe
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Nonlinear microscopy using impulsive stimulated Brillouin scattering for high-speed elastography
The impulsive stimulated Brillouin microscopy promises fast, non-contact measurements of the elastic properties of biological samples. The used pump-probe approach employs an ultra-short pulse laser and a cw laser to generate Brillouin signals.
Nektarios Koukourakis +2 more
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Dynamic Brillouin microscopy imaging
Optical Elastography and Tissue Biomechanics VII, 2020Brillouin microscopy is an emerging imaging modality in a broad area of biomedical research and clinical applications. Over the past decade, a significance progress has been achieved in developing better, more accurate and more user-friendly instrumentation for Brillouin microscopy and in fundamental understanding of the imaging contrast affordable in ...
Dominik Doktor +5 more
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Biophysical contrast mechanisms of Brillouin microscopy
Multimodal and Nanoscale Optical Microscopy, 2023Brillouin scattering spectroscopy, which for decades has been used in applied physics and material research, has recently gained prominence as a microscopy technique in biological research and clinical medicine for the measurement of mechanical properties of cells, tissues and biomaterials.
Fiore, Antonio, Scarcelli, Giuliano
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Brillouin microscopy for ocular biomechanics
Conference on Lasers and Electro-Optics 2010, 2010We present Brillouin confocal microscopy with high throughput and high extinction for non-invasive mechanical measurements of tissue and biomaterials. In particular, we demonstrate biomechanical characterization of crystalline lens and cornea.
Giuliano Scarcelli, Seok H. Yun
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Coaxial line scanning Brillouin microscopy
Optical Elastography and Tissue Biomechanics XIIAbstract Confocal Brillouin microscopy (CBM) enables high-resolution mechanical imaging but has slow acquisition speeds due to its point-by-point scanning strategy. Line-scanning Brillouin microscopy (LSBM) offers imaging acquisition speed improvements but faces challenges such as beam distortion in biaxial configurations and ...
Chenjun Shi, Jitao Zhang
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Noninvasive Imaging: Brillouin Confocal Microscopy
2018In the past decades, there has been increased awareness that mechanical properties of tissues and cells are closely associated with disease physiology and pathology. Recognizing this importance, Brillouin spectroscopy instrumentation, already utilized in physics and material science, has been adopted for cell and tissue biomechanics.
Miloš, Nikolić +3 more
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Brillouin Scanning Microscopy in Keratoconus
2016The mechanical properties of the cornea are thought to be central in the development of keratoconus and other corneal ectatic disorders. However, current technologies to measure mechanical properties are limited for in vivo and/or in situ applications. We have recently introduced a new approach, based on optical Brillouin scattering, toward developing ...
Giuliano Scarcelli, Seok Hyun Yun
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Brillouin microscopy for tissue and cell biomechanics
CLEO: 2015, 2015We have developed an all-optical approach to measure material mechanical properties using Brillouin light scattering. Brillouin imaging uses the elastic modulus as contrast mechanism. We demonstrate its application in vivo for tissue and cellular biomechanics.
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Line-scanning speeds up Brillouin microscopy
Nature Methods, 2023Nargess Khalilgharibi +2 more
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