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BSA-seq

This project performed a bulked segregant analysis sequencing (BSA-seq) strategy using an F2:3 segregation population to identify the candidate loci involved in verticillium wilt resistance of upland cotton, as well as enriches the genomic information and gene resources for the molecular breeding of disease resistance in cotton.
Jianbo Zhang   +2 more
openaire   +2 more sources

BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines

Molecular Breeding, 2019
© 2019, Springer Nature B.V. Broomrape (Orobanche cumana) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F 3 families were phenotyped for resistance to broomrape race G.
Ivana Imerovski   +7 more
openaire   +3 more sources

Fine-mapping of a major QTL controlling plant height by BSA-seq and transcriptome sequencing in cotton

Theoretical and Applied Genetics
GhSOT (GH_D05G3950) plays a negative role in regulating plant height development by modulating the GA signaling. Plant height is an important indicator affecting mechanical harvesting for cotton. Therefore, understanding the genes associated with the plant height is crucial for cotton breeding and production.
Chao Li   +6 more
openaire   +2 more sources

Integrated transcriptome and BSA-seq analysis identifies a novel QTL for Meloidogyne graminicola resistance in rice HuaHang31

Theoretical and Applied Genetics
A novel QTL on chromosome 11 with 7 candidate genes regulating nematode resistance was identified by RNA-seq-based transcriptome and BSA-seq combination analyses, revealing the possible interactions between nematodes and plants. The resistant rice germplasms against Meloidogyne graminicola, which is a plant-parasitic nematode posing a significant ...
Zhuhong Yang   +9 more
openaire   +2 more sources

BSA-seq and QTL Analysis of Triadimefon Resistance in Wheat Stripe Rust Fungus

To identify genetic loci underlying triadimefon resistance in Puccinia striiformis f. sp. tritici (Pst), we performed BSA-seq on F2 isolates (n=83) with divergent sensitivity. Genomic DNA from urediniospores (CTAB-extracted) was pooled into resistant/sensitive bulks (20 isolates each) and sequenced (Illumina paired-end).
openaire   +1 more source

Optimization of BSA-seq experiment for QTL mapping

G3: Genes, Genomes, Genetics, 2022
Weiren Wu, Weiqi Tang, P Ingvarsson
exaly  

Mining candidate genes underlying seed oil content using BSA-seq in soybean

Industrial Crops and Products, 2023
Shengrui Zhang   +17 more
openaire   +1 more source

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