Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution [PDF]
Nature CommunicationsThe placenta is a complex and heterogeneous organ that links the mother and fetus, playing a crucial role in nourishing and protecting the fetus throughout pregnancy. Integrative spatial multi-omics approaches can provide a systems-level understanding of
Marija Veličković +15 more
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Enabling malic acid production from corn-stover hydrolysate in Lipomyces starkeyi via metabolic engineering and bioprocess optimization [PDF]
Microbial Cell FactoriesBackground Lipomyces starkeyi is an oleaginous yeast with a native metabolism well-suited for production of lipids and biofuels from complex lignocellulosic and waste feedstocks.
Jeffrey J. Czajka +15 more
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Evaluation of chromosomal insertion loci in the Pseudomonas putida KT2440 genome for predictable biosystems design [PDF]
Metabolic Engineering Communications, 2020 The development of Pseudomonas strains for industrial production of fuels and chemicals will require the integration of heterologous genes and pathways into the chromosome.
Julie E. Chaves +13 more
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Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering [PDF]
Nature Communications, 2022 Muconic acid is a platform chemical with wide industrial applicability. Here, the authors report efficient muconate production from glucose and xylose by engineered Pseudomonas putida strain using adaptive laboratory evolution, metabolic modeling, and ...
Chen Ling +16 more
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Unfolded Protein Response Inhibition Reduces Middle East Respiratory Syndrome Coronavirus-Induced Acute Lung Injury [PDF]
mBio, 2021 Tissue- and cell-specific expression patterns are highly variable within and across individuals, leading to altered host responses after acute virus infection.
Amy C. Sims +13 more
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PeakDecoder enables machine learning-based metabolite annotation and accurate profiling in multidimensional mass spectrometry measurements [PDF]
Nature Communications, 2023 Multidimensional measurements using state-of-the-art separations and mass spectrometry provide advantages in untargeted metabolomics analyses for studying biological and environmental bio-chemical processes.
Aivett Bilbao +27 more
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The human plasma lipidome response to exertional heat tolerance testing [PDF]
Lipids in Health and DiseaseBackground The year of 2023 displayed the highest average global temperatures since it has been recorded—the duration and severity of extreme heat are projected to increase. Rising global temperatures represent a major public health threat, especially to
Igor L. Estevao +14 more
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Integration of Proteomics and Metabolomics Into the Design, Build, Test, Learn Cycle to Improve 3-Hydroxypropionic Acid Production in Aspergillus pseudoterreus [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Biological engineering of microorganisms to produce value-added chemicals is a promising route to sustainable manufacturing. However, overproduction of metabolic intermediates at high titer, rate, and yield from inexpensive substrates is challenging in ...
Kyle R. Pomraning +21 more
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High-Throughput Large-Scale Targeted Proteomics Assays for Quantifying Pathway Proteins in Pseudomonas putida KT2440 [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Targeted proteomics is a mass spectrometry-based protein quantification technique with high sensitivity, accuracy, and reproducibility. As a key component in the multi-omics toolbox of systems biology, targeted liquid chromatography-selected reaction ...
Yuqian Gao +40 more
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Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass [PDF]
Biotechnology for Biofuels, 2021 Background Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biofuels and bioproducts represents ...
James Kirby +21 more
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