Results 171 to 180 of about 31,009,639 (254)

Process Analytical Technology (PAT) for Continuous Purification of mRNA via Precipitation and Tangential Flow Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Continuous downstream processing of messenger ribonucleic acid (mRNA) therapeutics requires purification strategies that are scalable, robust, and capable of removing product‐ and process‐related impurities from in vitro transcription mixtures.
Rajan R. Bhawnani, Maria del Carme Pons Royo, Rohan Kadambi, Andrew Hatas, Steve Burcat, Tyler Arnold, Hasan Al‐Mahayni, Richard D. Braatz, Allan S. Myerson   +8 more
wiley   +1 more source

The versatility of the CHASE domain in one-component systems of c-di-GMP signal transduction from <i>P. aeruginosa</i>. [PDF]

J Bacteriol
Angeli S, Scribani-Rossi C, Colasanto G, Spizzichino S, Rosignoli S, Giorgi A, Cutruzzolà F, Paone A, Tramonti A, Contestabile R, Montoya R, O'Toole GA, Paiardini A, Rinaldo S.   +13 more
europepmc   +1 more source

Identification of Putative Cellulose Synthase Gene xcsA2 Required for c‐di‐GMP‐Induced Biofilm Formation and Cell Aggregation in Synechococcus elongatus PCC 7942

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Synechococcus elongatus PCC 7942 is a widely used model cyanobacterium for diverse research fields and for bioproduction applications owing to its ease of cultivation, genetic modification, and ability to fix CO2. We found that a laboratory strain of S. elongatus PCC 7942 expressing the ydeH gene from E.
Chihiro C. Yamaguchi, Jiro F. Mori, Shinsuke Kutsuna, Robert A. Kanaly   +3 more
wiley   +1 more source

Architecture and regulatory functions of c-di-GMP signaling in classical Bordetella species. [PDF]

FEMS Microbiol Rev
Vondrova D, Mugni SL, Blumenstein J, Kasiztky C, Sisti F, Fernández J, Kamanova J.   +6 more
europepmc   +1 more source

Efficient Cell Removal and Impurity Reduction for Poxvirus Purification in a Single Step Alluvial Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modified Vaccinia virus Ankara (MVA) is a potent vector for the development of vaccines and novel cancer treatment strategies. Since it is a non‐replicating virus in human recipients, high infective titers are required for therapeutic efficacy.
Linus G. Weber, Caroline Stephan, Georgios Makris, Leander John, Ingo Jordan, Michael W. Wolff   +5 more
wiley   +1 more source

Structural analyses uncover protease-adhesin interactions and c-di-GMP receptor regulation in sulfate-reducing bacteria. [PDF]

Nat Commun
Font ME, Karbelkar AA, Lormand JD, Mortensen S, García-García MJ, O'Toole GA, Sondermann H.   +6 more
europepmc   +1 more source

10‐Fold Improvement in Depth Filter Capacity During Integrated Dynamic Bioprocessing (IDB) Purification Through Depth Filter Cycling and Precipitate Control Strategy

Biotechnology and Bioengineering, EarlyView.
ABSTRACT During bsAb1 purification, precipitate was formed during neutralization of the affinity capture product. This precipitate was removed before loading onto the first polishing column in the Integrated Dynamic Bioprocessing (IDB) platform.
David Scherr, Ana Kanazir, Samik Das, Nikunj Sharda, Jan Martinek, David Nellis, Michael Mollet, Jon Coffman   +7 more
wiley   +1 more source

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

Dual-function enzyme acts as a global c-di-GMP sink and local anti sigma factor antagonist to drive cellular differentiation. [PDF]

PLoS Genet
Cınar Cakmak B, Saric JD, Wrede K, Holmes NA, Haist J, Schumacher MA, Buttner MJ, Tschowri N.   +7 more
europepmc   +1 more source

From pathogen to “living drug factory” innovative strategies and clinical translation of bacteria as programmable intelligent vectors for cancer therapy

BMEMat, EarlyView.
Engineered bacteria serve as smart, living therapeutics that target tumors, deliver drugs, and remodel immunosuppressive microenvironments. This review highlights their potential to potentiate immunotherapy, radiotherapy, and chemotherapy, offering a promising paradigm for precise and synergistic cancer treatment. Abstract The dynamic interplay between
Haonan Shen, Weichen Zhang, Gaoli Niu, Li Wang, Ruiyan Li, Yueyue Fan, Feixue Lu, Lingling Xu, Xiaoyuan Ji, Yong Kang   +9 more
wiley   +1 more source