Results 271 to 280 of about 470,032 (357)

Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies

Biotechnology Progress, EarlyView.
Abstract For bioprocesses producing live virus, such as enterovirus Coxsackievirus A21, viral titer (infectivity basis) decay rates can exceed 30% within a day. Consequently, harvest timing is paramount. To optimize titer at harvest, a continuous viral product titer model was generated to elucidate kinetics.
Katherine Forrester, Thomas R. Blanda, Marena Trauger, Rachel Thompson, Neil Templeton   +4 more
wiley   +1 more source

Insertion of a Divergent GAF-like Domain Defines a Novel Family of YcgR Homologues That Bind c-di-GMP in Leptospirales. [PDF]

ACS Omega
Visnardi AB, Ribeiro RA, de Souza AS, Churasacari Vinces TG, Llontop EE, de Almeida Ferrari AS, França Henrique PA, Valdivieso D, Sánchez-Limache DE, Silva GR, Soares EP, Santos TWC, Farah CS, Sassonia RC, Salinas RK, Guzzo CR, de Souza RF.   +16 more
europepmc   +1 more source

A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy

Biotechnology Progress, EarlyView.
Using online metabolite data from the first few days of a bioreactor run, the digital shadow can forecast the cell concentration in real‐time with an accuracy of 13% (standard deviation ≈ 6%). These predictions can guide the optimal harvest time of CAR T cells for subsequent re‐infusion into the patient.
Joseph R. Egan, Núria Marí‐Buyé, Elia Vallejo Benítez‐Cano, Miquel Costa, Linda Wanika, Michael J. Chappell, Ursula Schultz, Jelena Ochs, Manuel Effenberger, David Horna, Qasim Rafiq, Stephen Goldrick   +11 more
wiley   +1 more source

The c-di-GMP effector FleQ controls alginate production by repressing transcription of <i>algD</i> in <i>Azotobacter vinelandii</i>. [PDF]

Microbiology (Reading)
Barrios-Rafael VV, Ahumada-Manuel CL, Orgaz-Ramírez S, Nava-Galeana J, Guzmán J, Moreno S, Bustamante VH, Núñez C.   +7 more
europepmc   +1 more source

A recurrent neural network for soft sensor development using CHO stable pools in fed‐batch process for SARS‐CoV‐2 spike protein production as a vaccine antigen

Biotechnology Progress, EarlyView.
Abstract Fed‐batch recombinant therapeutic protein (RTP) production processes utilizing Chinese Hamster Ovary (CHO) cells can take a long period of time (>10 days). Within this period, not all critical features may be measured routinely, and in fact, some are only measured once the process is terminated, complicating decision making.
Sebastian‐Juan Reyes, Robert Voyer, Yves Durocher, Olivier Henry, Phuong Lan Pham   +4 more
wiley   +1 more source

Polyethylene terephthalate (PET) primary degradation products affect c-di-GMP-, cAMP-signaling, and quorum sensing (QS) in <i>Vibrio gazogenes</i> DSM 21264. [PDF]

Microbiol Spectr
Preuss L, Alawi M, Dumnitch A, Trinh L, Maison W, Burmeister N, Poehlein A, Daniel R, Vollstedt C, Streit WR.   +9 more
europepmc   +1 more source

Optimizing clarification processes in biopharmaceutical manufacturing through quality by design: Strategies, implications, and future prospects

Biotechnology Progress, EarlyView.
Abstract Biopharmaceutical manufacturing processes in which the product of interest is extracellularly expressed typically employ a clarification step following cell culture or fermentation. During clarification, crude cell culture fluid or fermentation broth is processed to remove insoluble solids, cells, debris, and other particulates, with the ...
Kyeong‐won Yeop, Hyun‐ju Nam, Chang‐jae Shim, So‐mi Yang, Hyo‐won Kim, Cheon Ik Park, Subhasis Banerjee, Yanglin Mok   +7 more
wiley   +1 more source

Deciphering ligand and metal ion dependent intricate folding landscape of Vc2 c-di-GMP riboswitch aptamer. [PDF]

Nucleic Acids Res
Shin JY, Choi SR, An SY, Bang KM, Song HK, Suh JY, Kim NK.   +6 more
europepmc   +1 more source

Photocaged Dinucleoside Tetraphosphates for Light‐Mediated Activation of 5′ Cap Interactions and Translation In Vitro

ChemistryEurope, EarlyView.
Photocaged dinucleoside tetraphosphates can be incorporated into mRNA by T7 RNA polymerase. Subsequent irradiation with light activates 5′ cap interactions and translation. These 5′ cap analogs are useful tools to probe the functions of naturally occurring dinucleoside tetraphosphates and their role in bacteria and mammalian cells.
Cedrik Kühling, Wiebke Teich, Bayram Terzi, Helena Schepers, Sabine Hüwel, Andrea Rentmeister   +5 more
wiley   +1 more source

A novel transcriptional regulator, CdeR, modulates the type III secretion system via c-di-GMP signaling in <i>Dickeya dadantii</i>. [PDF]

Microbiol Spectr
Ghasemi A, Yuan X, Yang C-H.
europepmc   +1 more source