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Dystrophin and functionally related proteins in the nematode Caenorhabditis elegans
Neuromuscular Disorders, 2002We investigated the function of dystrophin in the nematode Caenorhabditis elegans. Although nematodes and mammals diverged early in evolution, their muscles share many structural and molecular features, thus rendering C. elegans relevant as a model to study muscle function. Dystrophin, dystrobrevin, dystroglycans and several sarcoglycans have conserved
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Monitoring Protein Synthesis in Caenorhabditis elegans Using SILAC
2017The static levels of proteins are the net results of their production and clearance regulated by the activities of proteins involved in their synthesis, degradation, and transportation. Therefore, the information on the rates of protein synthesis and clearance is needed to understand the underlying dynamic nature of a proteome.
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Combined Nucleotide and Protein Extractions in Caenorhabditis elegans.
Journal of visualized experiments : JoVE, 2020A single biological sample holds a plethora of information, and it is now common practice to simultaneously investigate several macromolecules to capture a full picture of the multiple levels of molecular processing and changes between different conditions. This protocol presents the method of isolating DNA, RNA, and protein from the same sample of the
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