Results 81 to 90 of about 3,468,317 (412)
Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β‐carbonic anhydrase
Here, we performed a functional analysis of the fungal enzymes belonging to clade D of the β‐class carbonic anhydrase family (β‐D‐CA). The β‐D‐CAs in the basidiomycete Gloeophyllum trabeum and the ascomycete Trichoderma harzianum showed very low activity in the hydration of CO2 but exhibited high activity in the hydrolysis of carbonyl sulfide (COS ...
Ryuka Iizuka+6 more
wiley +1 more source
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley+2 more
wiley +1 more source
Detecting stoichiometry of macromolecular complexes in live cells using FRET
Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report
Manu Ben-Johny+2 more
doaj +1 more source
Calcium scoring, a process in which arterial calcifications are detected and quantified in CT, is valuable in estimating the risk of cardiovascular disease events. Especially when used to quantify the extent of calcification in the coronary arteries, it is a strong and independent predictor of coronary heart disease events.
arxiv
Dendritic spike induction of postsynaptic cerebellar LTP [PDF]
The architecture of parallel fiber (PF) axons contacting cerebellar Purkinje neurons (PNs) retains spatial information over long distances. PF synapses can trigger local dendritic calcium spikes, but whether and how this calcium signal leads to plastic ...
Kaspar E. Vogt, Marco Canepari
core +1 more source
Calcium is an essential plant nutrient. It is required for various structural roles in the cell wall and membranes, it is a counter-cation for inorganic and organic anions in the vacuole, and the cytosolic Ca2+ concentration ([Ca2+]cyt) is an obligate ...
P. White, M. Broadley
semanticscholar +1 more source
In modular polyketide synthases, the dehydratase (DH) domain catalyzes the dehydration reaction of the β‐hydroxyacyl unit attached to the cognate acyl carrier protein (ACP) domain. However, it is unclear how DH interacts with ACP during the reaction. In this study, we identified DH–ACP interface residues, providing the first detailed insights into DH ...
Kaede Kotagiri+8 more
wiley +1 more source
Proximal tubule (PT) transports most of the renal Ca2+, which was usually described as paracellular (passive). We found a regulated Ca2+ entry pathway in PT cells via the apical transient receptor potential canonical 3 (TRPC3) channel, which initiates ...
Samuel Shin+5 more
doaj
The safety considerations of food-based solutions for vitamin D deficiency prevention, such as fortification and supplementation, are critical. On the basis of collective data from 20 randomized controlled trials (RCTs) and 20 national healthy surveys ...
Folasade A. Adebayo+6 more
doaj +1 more source
Calcium binding activity of the epidermal growth factor-like domains of the apicomplexan microneme protein EtMIC4 [PDF]
Microneme proteins are secreted from apicomplexan parasites during invasion of host cells and they play crucial roles in parasite-host cell adhesion.
Gill, A C+4 more
core +1 more source