Results 111 to 120 of about 7,712,280 (384)
Cell Reports, 2015 Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging.
Rebecca W. Beerman +6 more
doaj +1 more source
Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator
Neuron, 2019 Methods for one-photon fluorescent imaging of calcium dynamics in vivo are popular due to their ability to simultaneously capture the dynamics of hundreds of neurons across large fields of view, at a low equipment complexity and cost.
O. Shemesh +30 more
semanticscholar +1 more source
DISCo: Deep Learning, Instance Segmentation, and Correlations for Cell Segmentation in Calcium Imaging [PDF]
International Conference on Medical Image Computing and Computer-Assisted Intervention, 2019 Calcium imaging is one of the most important tools in neurophysiology as it enables the observation of neuronal activity for hundreds of cells in parallel and at single-cell resolution.
E. Kirschbaum +2 more
semanticscholar +1 more source
Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions
FEBS Letters, EarlyView.We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva +10 more
wiley +1 more source
Songbird organotypic culture as an in vitro model for interrogating sparse sequencing networks [PDF]
, 2017 Sparse sequences of neuronal activity are fundamental features of neural circuit computation; however, the underlying homeostatic mechanisms remain poorly understood. To approach these questions, we have developed a method for cellular-resolution imaging
Blute, Todd +7 more
core +1 more source
Fast nonconvex deconvolution of calcium imaging data. [PDF]
Biostatistics, 2018 Calcium imaging data promises to transform the field of neuroscience by making it possible to record from large populations of neurons simultaneously. However, determining the exact moment in time at which a neuron spikes, from a calcium imaging data set,
S. Jewell +3 more
semanticscholar +1 more source
Imaging Calcium Sparks in Cardiac Myocytes [PDF]
, 2010 Calcium ions play fundamental roles in many cellular processes in virtually all type of cells. The use of Ca(2+) sensitive fluorescent indicators has proven to be an indispensable tool for studying the spatio-temporal dynamics of intracellular calcium ([Ca(2+)](i)).
Silvia, Guatimosim +2 more
openaire +2 more sources
FEBS Letters, EarlyView.Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson +7 more
wiley +1 more source
MIN1PIPE: A Miniscope 1-photon-based Calcium Imaging Signal Extraction Pipeline
bioRxiv, 2018 In vivo calcium imaging using 1-photon based miniscope and microendoscopic lens enables studies of neural activities in freely behaving animals. However, the high and fluctuating background, the inevitable movements and distortions of imaging field, and ...
Jinghao Lu +6 more
semanticscholar +1 more source
β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1
Molecular Oncology, EarlyView.Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero +8 more
wiley +1 more source