Results 91 to 100 of about 636,488 (297)
The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes
FEBS Letters, EarlyView.Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga +3 more
wiley +1 more source
A simple method to reconstruct firing rates from dendritic calcium signals
Frontiers in Neuroscience, 2008 Calcium imaging using fluorescent reporters is the most widely used optical approach to investigate activity in intact neuronal circuits with single-cell resolution.
Laurent C Moreaux +2 more
doaj +1 more source
Automatic Neuron Detection in Calcium Imaging Data Using Convolutional Networks
, 2016 Calcium imaging is an important technique for monitoring the activity of thousands of neurons simultaneously. As calcium imaging datasets grow in size, automated detection of individual neurons is becoming important.
Aguilar, Rob E. +6 more
core
FEBS Letters, EarlyView.Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley +1 more source
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue.
PLoS ONE, 2016 Calcium-imaging is a sensitive method for monitoring calcium dynamics during neuronal activity. As intracellular calcium concentration is correlated to physiological and pathophysiological activity of neurons, calcium imaging with fluorescent indicators ...
Morven Cameron +6 more
doaj +1 more source
Network Properties Revealed during Multi-Scale Calcium Imaging of Seizure Activity in Zebrafish. [PDF]
, 2019 Seizures are characterized by hypersynchronization of neuronal networks. Understanding these networks could provide a critical window for therapeutic control of recurrent seizure activity, i.e., epilepsy.
Baraban, Scott C, Liu, Jing
core +1 more source
AAA+ protein unfoldases—the Moirai of the proteome
FEBS Letters, EarlyView.AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley +1 more source
BMC Cancer, 2017 Background The tumor-derived GL261 cell line is used as a model for studying glioblastoma and other high-grade gliomas, and can be cultured adherently or as free-floating aggregates known as neurospheres.
Averey D. Strong, Richard L. Daniels
doaj +1 more source
Large-scale imaging of brain network activity from >10,000 neocortical cells [PDF]
, 2009 Large-scale recording from populations of neurons is a promising strategy in the study of complex brain function. Here we introduce a simple optical technique that simultaneously probes the calcium activity of ~10,000 cells at the single cell resolution ...
Norio Matsuki +2 more
core +1 more source
Molecular Oncology, EarlyView.Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice +16 more
wiley +1 more source