A new phase for two-photon microscopy [PDF]
Frontiers in Neuroscience, 2009 Two-photon excitation has enabled remarkable advances in our understanding of the nervous system. Its three-dimensional resolution, superior depth penetration, and minimal phototoxicity for out-of-focus portions of the sample have made it the technique of choice for an impressive array of applications.
doaj +3 more sources
Two-Photon Microscopy of Cells and Tissue [PDF]
Circulation Research, 2004 Two-photon excitation fluorescence imaging provides thin optical sections from deep within thick, scattering specimens by way of restricting fluorophore excitation (and thus emission) to the focal plane of the microscope. Spatial confinement of two-photon excitation gives rise to several advantages over single-photon confocal microscopy.
Michael Rubart
exaly +3 more sources
Two-photon microscopy for microrobotics: Visualization of micro-agents below fixed tissue. [PDF]
PLoS ONE, 2023 Optical microscopy is frequently used to visualize microrobotic agents (i.e., micro-agents) and physical surroundings with a relatively high spatio-temporal resolution.
Juan J Huaroto +7 more
doaj +2 more sources
MEMS Enabled Miniature Two-Photon Microscopy for Biomedical Imaging [PDF]
Micromachines, 2023 Over the last decade, two-photon microscopy (TPM) has been the technique of choice for in vivo noninvasive optical brain imaging for neuroscientific study or intra-vital microendoscopic imaging for clinical diagnosis or surgical guidance because of its ...
Xiaomin Yu +4 more
doaj +2 more sources
Intravital two-photon microscopy of the native mouse thymus. [PDF]
PLoS ONEThe thymus, a key organ in the adaptive immune system, is sensitive to a variety of insults including cytotoxic preconditioning, which leads to atrophy, compression of the blood vascular system, and alterations in hemodynamics.
Negar Seyedhassantehrani +5 more
doaj +2 more sources
Needle beam two-photon microscopy for simultaneous multiplane neural and vascular imaging in awake mice [PDF]
PhotoniXComprehensive understanding of brain functions necessitates high-speed imaging of neuronal and vascular dynamics across extensive volumes. Functional neuroimaging investigations with two-photon microscopy are commonly hindered by its limited depth of ...
Quanyu Zhou +11 more
doaj +2 more sources
The Micro-Structure of the Celiac Ganglia—A Two-Photon Microscopy Study on Parkinson’s Disease [PDF]
DiagnosticsBackground/Objectives: This study explores the micro-structure of celiac ganglia using two-photon microscopy (TPM) to highlight histological features in neurodegenerative conditions.
Diana-Theodora Morgos +9 more
doaj +2 more sources
Insight into pericytes in glioblastoma angiogenesis: In vivo tracking by two‐photon microscopy and proteomic profiling [PDF]
Animal Models and Experimental MedicineBackground Glioblastoma (GBM) is a highly aggressive brain tumor characterized by aberrant angiogenesis and an immunosuppressive microenvironment. Pericytes are aberrantly recruited but their spatiotemporal roles and molecular changes remain unclear ...
Qinghong Wang +5 more
doaj +2 more sources
Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy
Photonics, 2023 We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon ...
Colin J. R. Sheppard +7 more
doaj +1 more source
Two-Photon Imaging to Unravel the Pathomechanisms Associated with Epileptic Seizures: A Review
Applied Sciences, 2021 Despite extensive research, the exact pathomechanisms associated with epileptic seizure formation and propagation have not been elucidated completely. Two-photon imaging (2PI) is a fluorescence-based microscopy technique that, over the years, has been ...
Luqman Khan +7 more
doaj +1 more source