Results 41 to 50 of about 288,583 (303)
Wide-field two-photon microscopy with temporal focusing and HiLo background rejection [PDF]
, 2019 Scanningless depth-resolved microscopy is achieved through spatial-temporal focusing and has been demonstrated previously. The advantage of this method is that a large area may be imaged without scanning resulting in higher throughput of the imaging ...
So, Peter T. C. +3 more
core +1 more source
Two-photon microscopy : sequential imaging studies in vivo [PDF]
, 2011 Microscopists have always desired to look inside various organ tissues to study structure, function and dysfunction of their cellular constituents. In the past, this has frequently required tissue extraction and histological preparation to gain access ...
Zammit, Christian +4 more
core
Molecular Oncology, EarlyView.We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric +10 more
wiley +1 more source
Scientific Reports, 2021 Fluoro-Gold is a fluorescent neuronal tracer suitable for targeted deep imaging of the nervous system. Widefield fluorescence microscopy enables visualization of Fluoro-Gold, but lacks depth discrimination.
Matthew Q. Miller +4 more
doaj +1 more source
Frontiers in Immunology, 2022 BackgroundResults from our recent study demonstrate that sphingosine-1-phosphate receptor 1 (S1PR1) modulation improves microvascular hemodynamics after cerebrovascular thrombosis.
Han-Dong Li +7 more
doaj +1 more source
Two-photon excitation STED microscopy
Optics Express, 2009 We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution
Moneron, G., Hell, S.
openaire +4 more sources
Molecular Oncology, EarlyView.We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober +16 more
wiley +1 more source
Cranial irradiation disrupts homeostatic microglial dynamic behavior
Journal of NeuroinflammationCranial irradiation causes cognitive deficits that are in part mediated by microglia, the resident immune cells of the brain. Microglia are highly reactive, exhibiting changes in shape and morphology depending on the function they are performing ...
Alexandra O. Strohm +5 more
doaj +1 more source
Clinical Ophthalmology, 2020 Thales Antonio Abra De Paula, Fernando Betty Cresta, Milton Ruiz Alves University of São Paulo USP, Ophthalmology, Medical School, São Paulo, Brazil Correspondence: Thales Antonio Abra De Paula Rua Apeninos 800,apt 1004, São Paulo,
De Paula TAA, Cresta FB, Alves MR
doaj
Applications of two-photon microscopy in the neurosciences
Frontiers in Bioscience, 2005 Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give
Cruz, H. G., Luescher, Christian
openaire +4 more sources