Results 41 to 50 of about 20,254,963 (292)

Important characteristics of two-photon intravital microscopy

open access: yes, 2023
Multiphoton microscopy techniques find a unique niche in intravital microscopy where whole animals could be imaged up to a tissue depth of 1 mm. However, there remains critical details of the technique that remain subject of academic debate.
Wenfa Ng (9593279)
core   +1 more source

Rapid and label-free detection of gastrointestinal stromal tumor via a combination of two-photon microscopy and imaging analysis

open access: yesBMC Cancer, 2023
Background Gastrointestinal stromal tumor (GIST) is currently regarded as a potentially malignant tumor, and early diagnosis is the best way to improve its prognosis.
Lianhuang Li   +8 more
doaj   +1 more source

Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. [PDF]

open access: yes, 2011
Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen.
Krombach Fritz   +11 more
core   +2 more sources

Two-Photon Imaging

open access: yes, 2022
This chapter will provide an overview of two-photon microscopy from elements of the theory underpinning fluorescence phenomena to functioning principles of a two-photon microscope including step-by-step practical advice on how to conduct an experiment ...
Sancataldo G., Barrera O., Vetri V.
core   +1 more source

Two-photon excitation STED microscopy

open access: yesOptics Express, 2009
We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution
Moneron, Gael, Hell, Stefan
openaire   +4 more sources

Two-Photon Fluorescence Microscopy Imaging of Cellular Oxidative Stress Using Profluorescent Nitroxides [PDF]

open access: yes, 2012
A range of varying chromophore nitroxide free radicals and their nonradical methoxyamine analogues were synthesized and their linear photophysical properties examined.
Fairfull-Smith, Kathryn E.   +8 more
core   +1 more source

Selective Sphingosine 1-Phosphate Receptor 1 Modulation Augments Thrombolysis of Low-Dose Tissue Plasminogen Activator Following Cerebrovascular Thrombosis

open access: yesFrontiers in Immunology, 2022
BackgroundResults from our recent study demonstrate that sphingosine-1-phosphate receptor 1 (S1PR1) modulation improves microvascular hemodynamics after cerebrovascular thrombosis.
Han-Dong Li   +7 more
doaj   +1 more source

Applications of two-photon microscopy in the neurosciences

open access: yesFrontiers in Bioscience, 2005
Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give
Cruz, H. G., Luescher, Christian
openaire   +5 more sources

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

open access: yesMolecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober   +16 more
wiley   +1 more source

Two-photon microscopy : sequential imaging studies in vivo [PDF]

open access: yes, 2011
Microscopists have always desired to look inside various organ tissues to study structure, function and dysfunction of their cellular constituents. In the past, this has frequently required tissue extraction and histological preparation to gain access ...
Zammit, Christian   +4 more
core  

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