Results 61 to 70 of about 20,254,963 (292)
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Intravital imaging of megakaryocytes
Platelets, 2020 The dynamics of platelet formation could only be investigated since the development of two-photon microscopy in combination with suitable fluorescent labeling strategies.
David Stegner, Katrin G. Heinze
doaj +1 more source
Frontiers in Microbiology, 2021 Kombucha pellicles are often used as inoculum to produce this beverage and have become a signature feature. This cellulosic biofilm produced by acetic acid bacteria (AAB) involves yeasts, which are also part of the kombucha consortia.
Thierry Tran +7 more
doaj +1 more source
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
FEBS Open Bio, EarlyView.Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source
Wide-field two-photon microscopy with temporal focusing and HiLo background rejection [PDF]
, 2011 Scanningless depth-resolved microscopy is achieved through spatial-temporal focusing and has been demonstrated previously. The advantage of this method is that a large area may be imaged without scanning resulting in higher throughput of the imaging ...
So, Peter T. C. +7 more
core +1 more source
Structure and Sedimentation Kinetics of Dense Suspensions of Fibroblast Cells
CHIMIA, 2019 : We investigate the structure and the dynamics of dense suspensions of NIH 3T3 fibroblast cells. Using two-photon microscopy we obtain three dimensional (3D) images from which the size and the packing structure of the dense cell suspensions can be ...
Arbnor Zenuni +4 more
doaj +1 more source
Cardiovascular Imaging Using Two-Photon Microscopy [PDF]
Microscopy and Microanalysis, 2008 AbstractTwo-photon excitation microscopy has become the standard technique for high resolution deep tissue and intravital imaging. It provides intrinsic three-dimensional resolution in combination with increased penetration depth compared to single-photon confocal microscopy.
John A, Scherschel, Michael, Rubart
openaire +2 more sources
Hydrogel platforms for 3D cardiac tissue engineering and fibrotic disease modelling
FEBS Open Bio, EarlyView.Hydrogel‐based cardiac tissue models are advancing our understanding of heart health and disease. By incorporating electrical conductivity, aligned structures, and tunable mechanical properties, these platforms better replicate the complex environment of native heart tissue.
Harrison Porritt +2 more
wiley +1 more source
eLife, 2016 Axon guidance is proposed to act through a combination of long- and short-range attractive and repulsive cues. The ligand-receptor pair, Netrin (Net) and Frazzled (Fra) (DCC, Deleted in Colorectal Cancer, in vertebrates), is recognized as the ...
Orkun Akin, S Lawrence Zipursky
doaj +1 more source
Two-photon intravital imaging of leukocyte migration during inflammation in the respiratory system [PDF]
Acute and Critical Care, 2019 Two-photon intravital imaging is a powerful method by which researchers are able to directly observe biological phenomena in live organisms. Researchers in various biomedical research fields have applied two-photon imaging to a variety of target organs ...
Young Min Kim +3 more
doaj +1 more source