Deep tissue imaging with multiphoton fluorescence microscopy [PDF]
Current Opinion in Biomedical Engineering, 2017 We present a review of imaging deep-tissue structures with multiphoton microscopy. We examine the effects of light scattering and absorption due to the optical properties of biological sample and identify 1,300 nm and 1,700 nm as ideal excitation ...
David R Miller +2 more
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Scanless multitarget-matching multiphoton excitation fluorescence microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2018 Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved.
Junpeng Qiu +4 more
doaj +5 more sources
A dataset of rodent cerebrovasculature from in vivo multiphoton fluorescence microscopy imaging
Scientific Data, 2023 We present MiniVess, the first annotated dataset of rodent cerebrovasculature, acquired using two-photon fluorescence microscopy. MiniVess consists of 70 3D image volumes with segmented ground truths.
Charissa Poon +4 more
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Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Molecules, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Gregor P. C. Drummen +2 more
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Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates [PDF]
Nature Communications, 2020 Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear
Sebastian Karpf +7 more
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Recent progress of second near-infrared (NIR-II) fluorescence microscopy in bioimaging
Frontiers in Physiology, 2023 Visualizing biological tissues in vivo at a cellular or subcellular resolution to explore molecular signaling and cell behaviors is a crucial direction for research into biological processes.
Tian Wang +3 more
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Fluorescence Correlation Spectroscopy Combined with Multiphoton Laser Scanning Microscopy—A Practical Guideline [PDF]
Applied Sciences, 2021 Multiphoton laser scanning microscopy (MPM) has opened up an optical window into biological tissues; however, imaging is primarily qualitative. Cell morphology and tissue architectures can be clearly visualized but quantitative analysis of actual ...
Jeemol James +2 more
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Uses of coherent Raman scattering microscopy in neuroscience [PDF]
Frontiers in NeuroscienceMultiphoton microscopy allows the imaging of biological phenomena deep within brain tissues and has greatly advanced knowledge in neuroscience. However, many optical phenomena other than the multiphoton excitation of fluorophores in nonlinear optics are ...
Mutsuo Nuriya, Mutsuo Nuriya
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Challenges and Prospects of Using Novel Nonlinear Effects in Multimode Optical Fibers for Multiphoton Endomicroscopy [PDF]
DiagnosticsMultiphoton endomicroscopy (MPEM) has recently become a key development in optical biomedical diagnostics, providing histologically relevant in vivo images that are eliminating both the need for tissue damage during biopsy sampling and the need for dye ...
Lidiya V. Boldyreva +12 more
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Evaluation of a nanosecond dissipative soliton resonance fiber laser for multiphoton microscopy [PDF]
Scientific ReportsMultiphoton microscopy often relies on femtosecond lasers due to their high peak powers and short pulse durations. However, these systems (e.g., Ti:Sapphire) are expensive, complex, and require dispersion compensation to maintain pulse parameters in the ...
Katarzyna Kunio +4 more
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