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Multiphoton Microscopy and Fluorescence Lifetime Imaging [PDF]

, 2018
This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences.

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Multiphoton Fluorescence Microscopy [PDF]

Methods, 2001
Multiphoton fluorescence microscopy has now become a relatively common tool among biophysicists and biologists. The intrinsic sectioning achievable by multiphoton excitation provides a simple means to excite a small volume inside cells and tissues ...
Barry, Nicholas P, Gratton, Enrico, Beretta, Sabrina, Celli, Anna   +3 more
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Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin [PDF]

Dermatology Research and Practice, 2012
Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity
Stefania Seidenari, Federica Arginelli, Sara Bassoli, Jennifer Cautela, Paul M. W. French, Mario Guanti, Davide Guardoli, Karsten König, Clifford Talbot, Chris Dunsby   +9 more
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Characterization of Pancreatic Cancer Tissue Using Multiphoton Excitation Fluorescence and Polarization-Sensitive Harmonic Generation Microscopy

Frontiers in Oncology, 2019
Thin tissue sections of normal and tumorous pancreatic tissues stained with hematoxylin and eosin were investigated using multiphoton excitation fluorescence (MPF), second harmonic generation (SHG), and third harmonic generation (THG) microscopies.
Danielle Tokarz, Richard Cisek, Ariana Joseph, Ahmad Golaraei, Ahmad Golaraei, Ahmad Golaraei, Kamdin Mirsanaye, Kamdin Mirsanaye, Serguei Krouglov, Serguei Krouglov, Sylvia L. Asa, Brian C. Wilson, Brian C. Wilson, Virginijus Barzda, Virginijus Barzda   +14 more
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Fluorescence Signal Generation Optimization by Optimal Filling of the High Numerical Aperture Objective Lens for High-Order Deep-Tissue Multiphoton Fluorescence Microscopy

IEEE Photonics Journal, 2015
Deep-tissue multiphoton microscopy (MPM) enables noninvasive optical imaging into the deep regions of the tissue in animal models in vivo. High-order MPM techniques, such as three- and four-photon fluorescence microscopy at the 1700-nm window, are ...
Ke Wang, Runfu Liang, Ping Qiu
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Multiphoton microscopy imaging of fibrous meningiomas based on the combination of multichannel mode and lambda mode [PDF]

Frontiers in Neuroscience
Fibrous meningiomas, known for their dense and tough texture, present unique challenges in diagnosis and surgical treatment. This study explores the potential of multiphoton microscopy (MPM) for visualizing the microstructures of fibrous meningiomas by ...
Linghan You, Linjing Shi, Yuqing Huang, Lingxin Pan, Shiying Zheng, Yingyuan Wang, Zanyi Wu, Xingfu Wang, Jianxin Chen, Na Fang   +9 more
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The effects of spherical aberration on multiphoton fluorescence excitation microscopy [PDF]

Journal of microscopy, 2011
Multiphoton fluorescence excitation microscopy is almost invariably conducted with samples whose refractive index differ from that of the objective immersion medium, conditions that cause spherical aberration.
Byars, J. M., Dunn, K. W., Young, P. A., Clendenon, S. G., Decca, R. S.   +4 more
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Multiphoton excitation fluorescence microscopy of 5-aminolevulinic acid induced fluorescence in experimental gliomas

Lasers in Surgery and Medicine, 2008
Background and Objective: The clinical usefulness of 5-ALA guided detection of tumor tissue has been demonstrated for a number of malignancies. However, current techniques of intraoperative detection of protoporphyrin IX fluorescence in situ do not offer
Diddens, Heike, Leppert, Jan, Rohde, Veit, Kantelhardt, Sven Rainer, Huettmann, Gereon, Giese, Alf   +5 more
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The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging

International Journal of Molecular Sciences, 2023
A decrease in the regenerative potential of the liver during the development of non-alcoholic fatty liver disease (NAFLD), which is observed in the vast majority of patients with diabetes mellitus type 1, significantly increases the risk of postoperative
S. Rodimova, N. Bobrov, Artem M. Mozherov, V. Elagin, M. Karabut, P. Ermakova, Ilya D Shchechkin, Dmitry Kozlov, D. Krylov, A. Gavrina, A. Kashina, Vladimir E Zagainov, E. Zagaynova, D. Kuznetsova   +13 more
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Insights into Metabolic Activity and Structure of the Retina through Multiphoton Fluorescence Lifetime Imaging Microscopy in Mice

Cells, 2022
Fluorescence lifetime imaging microscopy (FLIM) evaluates the metabolic state of tissue based on reduced nicotinamide adenine dinucleotide (NAD(P)H) and flavin adenine dinucleotide (FAD).
Niranjana Kesavamoorthy, Jason A. Junge, S. Fraser, Hossein Ameri   +3 more
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