Results 31 to 40 of about 160,406 (282)

Superficial Nephrons in BALB/c and C57BL/6 Mice Facilitate In Vivo Multiphoton Microscopy of the Kidney [PDF]

open access: yes, 2013
Multiphoton microscopy (MPM) offers a unique approach for addressing both the function and structure of an organ in near-real time in the live animal. The method however is limited by the tissue-specific penetration depth of the excitation laser.
Bardehle, Sophia   +11 more
core   +1 more source

Fluorescence Lifetime Imaging Microscopy (FLIM) reveals spatial-metabolic changes in 3D breast cancer spheroids

open access: yesScientific Reports, 2023
Cancer cells are mechanically sensitive to physical properties of the microenvironment, which can affect downstream signaling to promote malignancy, in part through the modulation of metabolic pathways. Fluorescence Lifetime Imaging Microscopy (FLIM) can
Kavon Karrobi   +7 more
semanticscholar   +1 more source

Multiphoton fluorescence microscopy of the live kidney in health and disease [PDF]

open access: yesJournal of Biomedical Optics, 2014
The structural and functional heterogeneity of the kidney ensures a diversity of response in health and disease. Multiphoton microscopy has improved our understanding of kidney physiology and pathophysiology by enabling the visualization of the living kidney in comparison with the static view of previous technologies.
Small, David M.   +4 more
openaire   +5 more sources

Measuring enzyme binding using shaped ultrafast laser pulses

open access: yesEPJ Web of Conferences, 2013
We use multiphoton quantum-control spectroscopy to discriminate between enzyme-bound and unbound NADH (reduced nicotinamide adenine dinucleotide) molecules in solution.
Weinacht T. C.   +2 more
doaj   +1 more source

Super resolution measurement of collagen fibers in biological samples: Validation of a commercial solution for multiphoton microscopy.

open access: yesPLoS ONE, 2020
Multiphoton microscopy is a powerful, non-invasive technique to image biological specimens. One current limitation of multiphoton microscopy is resolution as many of the biological molecules and structures investigated by research groups are similar in ...
Aaron M Barlow   +4 more
doaj   +1 more source

Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy [PDF]

open access: yesScientific Reports, 2011
Fast three-(3D) imaging requires parallel optical slicing of a specimen with an efficient detection scheme. The generation of multiple localized dot-like excitation structures solves the problem of simultaneous slicing multiple specimen layers, but an efficient detection scheme is necessary.
Mondal P. P., DIASPRO, ALBERTO GIOVANNI
openaire   +3 more sources

Intrinsic indicator of photodamage during label-free multiphoton microscopy of cells and tissues.

open access: yesPLoS ONE, 2014
Multiphoton imaging has evolved as an indispensable tool in cell biology and holds prospects for clinical applications. When addressing endogenous signals such as coherent anti-Stokes Raman scattering (CARS) or second harmonic generation, it requires ...
Roberta Galli   +7 more
doaj   +1 more source

Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm

open access: yesIEEE Photonics Journal, 2018
Near infrared (NIR) excitation or emission is capable of deep-tissue penetration in various modalities of optical microscopy. Based on the transmittance characterization of biological tissue, such as brains, four NIR optical windows have been ...
Ke Wang   +5 more
doaj   +1 more source

Multiphoton FLIM and spectral imaging of cells and tissues

open access: yes, 2022
S.240-251Five-dimensional (5D) multiphoton measurements with submicron spatial resolution, 270 ps temporal resolution and 5 nm spectral resolution have been performed on living cells and tissues at 750 nm - 850 nm laser excitation. A compact (65×62×48 cm(
Ehrlich, G.   +4 more
core   +1 more source

Advances in Ultrafast Fiber Lasers for Multiphoton Microscopy in Neuroscience

open access: yesPhotonics, 2023
Multiphoton microscopy (MPM) has emerged as a vital tool in neuroscience, enabling deeper imaging with a broader field of view, as well as faster and sub-cellular resolution.
T. Srinivasan, Murat Yildirim
semanticscholar   +1 more source

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