Results 21 to 30 of about 160,406 (282)

Multiphoton Excitation Fluorescence Microscopy and Spectroscopic Multianalytical Approach for Characterization of Historical Glass Grisailles.

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2021
The preservation of the integrity of artworks and cultural heritage items during characterization and conservation operations is of high priority, therefore, the application of non-invasive techniques is commonly suggested and recommended.
M. Oujja, F. Agua, M. Sanz, D. Morales-Martín, M. García-Heras, M. Villegas, M. Castillejo   +6 more
semanticscholar   +1 more source

Diamond Raman Laser and Yb Fiber Amplifier for In Vivo Multiphoton Fluorescence Microscopy

bioRxiv, 2021
Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, 1250 nm) for multiphoton excitation of red-shifted fluorophores.
Shaun A. Engelmann, Annie Zhou, Ahmed M. Hassan, Michael R. Williamson, J. W. Jarrett, E. Perillo, Alankrit Tomar, D. Spence, T. A. Jones, A. Dunn   +9 more
semanticscholar   +1 more source

Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging. [PDF]

PLoS ONE, 2016
We derive a physically realistic model for the generation of virtual transillumination, white light microscopy images using epi-fluorescence measurements from thick, unsectioned tissue.
Michael G Giacomelli, Lennart Husvogt, Hilde Vardeh, Beverly E Faulkner-Jones, Joachim Hornegger, James L Connolly, James G Fujimoto   +6 more
doaj   +1 more source

Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae

Scientific Reports, 2021
Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy.
Dimitrios Kapsokalyvas, Rodrigo Rosas, Rob W. A. Janssen, Jo M. Vanoevelen, Miranda Nabben, Martin Strauch, Dorit Merhof, Marc A. M. J. van Zandvoort   +7 more
doaj   +1 more source

Intravital Monitoring of Psoriasis Treatment Response and Drug Delivery Using Multiphoton Fluorescence Lifetime Imaging. [PDF]

Skin Res Technol
ABSTRACT Background Multiphoton tomography equipped with fluorescence lifetime imaging (MPT‐FLIM) is a novel noninvasive imaging technique for analyzing morphological and metabolic states of skin diseases at a subcellular resolution. The present study is the first to establish MPT‐FLIM as an imaging modality to monitor treatment response in psoriasis ...
Nguyen L, Mess C, Herberger K, Schneider SW, Huck V.   +4 more
europepmc   +2 more sources

Evaluation of a gain-managed nonlinear fiber amplifier for multiphoton microscopy.

Biomedical Optics Express, 2023
Two-photon excited fluorescence microscopy is a widely-employed imaging technique that enables the noninvasive study of biological specimens in three dimensions with sub-micrometer resolution.
P. Sidorenko, M. Buttolph, M. Mejooli, Chi-yong Eom, C. Schaffer, F. Wise   +5 more
semanticscholar   +1 more source

Investigating biochemical and structural changes of glycated collagen using multimodal multiphoton imaging, Raman spectroscopy, and atomic force microscopy

Analytical and Bioanalytical Chemistry, 2023
Advanced glycation end products (AGEs) form extracellular crosslinking with collagenous proteins, which contributes to the development of diabetic complications. In this study, AGEs-related pentosidine (PENT) crosslinks-induced structural and biochemical
Elsie Quansah, T. Shaik, Ecehan Çevik, Xinyue Wang, C. Höppener, Tobias Meyer‐Zedler, V. Deckert, Michael Schmitt, J. Popp, C. Krafft   +9 more
semanticscholar   +1 more source

Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. [PDF]

, 2011
Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen.
Krombach Fritz, Rehberg, Markus, Fritz Krombach, Steffen Dietzel, Rehberg Markus, Pohl Ulrich, Dietzel, Steffen, Dietzel Steffen, Markus Rehberg, Ulrich Pohl, Pohl, Ulrich, Krombach, Fritz   +11 more
core   +1 more source

Report on fluorescence lifetime imaging using multiphoton laser scanning microscopy targeting sentinel lymph node diagnostics

Journal of Biomedical Optics, 2020
. Significance: Sentinel lymph node (SLN) biopsy is an important method for metastasis staging in, e.g., patients with malignant melanoma. Tools enabling prompt histopathological analysis are expected to facilitate diagnostics; optical technologies are ...
Jeemol James, Despoina Kantere, J. Enger, Jan Siarov, A. Wennberg, M. Ericson   +5 more
semanticscholar   +1 more source

Applications of combined spectral lifetime microscopy for biology

BioTechniques, 2006
Live cell imaging has been greatly advanced by the recent development of new fluorescence microscopy-based methods such as multiphoton laser-scanning microscopy, which can noninvasively image deep into live specimens and generate images of extrinsic and ...
Long Yan, Curtis T. Rueden, John G. White, Kevin W. Eliceiri   +3 more
doaj   +1 more source