Imaging microglia surveillance during sleep-wake cycles in freely behaving mice
eLife, 2023 Microglia surveillance manifests itself as dynamic changes in cell morphology and functional remodeling. Whether and how microglia surveillance is coupled to brain state switches during natural sleep-wake cycles remains unclear. To address this question,
Xiaochun Gu +11 more
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Study of dendritic spine compartmentalization : a correlative fluorescence light microscopy-electron microscopy approach [PDF]
, 2014 Neurons communicate with each other through synapses. Most excitatory synapses contact small protrusions called dendritic spines. Spines are connected to dendrites by a very thin stalk called the “spine neck” which restricts diffusion between the spine ...
Cyprien, Vivien
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Two-photon microscopy in pulmonary research [PDF]
Seminars in Immunopathology, 2010 As the lung is constantly exposed to both innocuous and potentially noxious antigens, a thorough understanding of both innate and adaptive immune responses in this organ is of the essence. Imaging modalities such as magnetic resonance imaging, positron emission tomography, and confocal microscopy have expanded our knowledge about various molecular ...
Ruben G, Nava +5 more
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Adaptive Optics in Two-Photon Microscopy and Two-Photon Lithography
, 2018 Two-photon microscopy has become an indispensable tool in biological research allowing imaging of intracellular structures especially deep in tissue. Due to its capability of three-dimensional (3D) fabrication in lithography, two-photon excitation is also an essential tool for nanofabrication.
Neuwirth, Julia
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Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. [PDF]
, 2011 Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen.
Krombach Fritz +11 more
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Pulse-shaping-based two-photon FRET microscopy
EPJ Web of Conferences, 2013 We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.
Swanson J. A. +4 more
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Improving Focal Photostimulation of Cortical Neurons with Pre-derived Wavefront Correction
Frontiers in Cellular Neuroscience, 2017 Recent progress in neuroscience to image and investigate brain function has been made possible by impressive developments in optogenetic and opto-molecular tools.
Vincent R. Daria +6 more
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Encoded multisite two-photon microscopy [PDF]
Proceedings of the National Academy of Sciences, 2013 The advent of scanning two-photon microscopy (2PM) has created a fertile new avenue for noninvasive investigation of brain activity in depth. One principal weakness of this method, however, lies with the limit of scanning speed, which makes optical interrogation of action potential-like activity in a neuronal network problematic.
Mathieu, Ducros +4 more
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Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard +5 more
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Two photon excitable graphene quantum dots for structured illumination microscopy and imaging application: lysosome specificity and tissue-dependent imaging [PDF]
, 2018 Two-photon active Graphene Quantum Dots (GQDs) are obtained from extracts of the neem root. These biocompatible GQDs are found to be suitable for structured illumination microscopy. Two-photon microscopy ensured lysosome specificity of GQDs in live cells
Sreedharan, S. +16 more
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