Results 11 to 20 of about 288,583 (303)

Photobleaching in Two-Photon Excitation Microscopy [PDF]

Biophysical Journal, 2000
The intensity-squared dependence of two-photon excitation in laser scanning microscopy restricts excitation to the focal plane and leads to decreased photobleaching in thick samples. However, the high photon flux used in these experiments can potentially lead to higher-order photon interactions within the focal volume.
Patterson, George H., Piston, David W.
openaire   +3 more sources

Comparing confocal and two-photon Ca2+ imaging of thin low-scattering preparations

Biophysical Reports, 2023
Ca2+ imaging provides insight into biological processes ranging from subcellular dynamics to neural network activity. Two-photon microscopy has assumed a dominant role in Ca2+ imaging.
Jinbo Cheng, Shane M. McMahon, David W. Piston, Meyer B. Jackson   +3 more
doaj   +1 more source

ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION [PDF]

Journal of Innovative Optical Health Sciences, 2013
Understanding brain structure and function, and the complex relationships between them, is one of the grand challenges of contemporary sciences. Thanks to their flexibility, optical techniques could be the key to explore this complex network.
L. SILVESTRI, A. L. ALLEGRA MASCARO, J. LOTTI, L. SACCONI, F. S. PAVONE   +4 more
doaj   +1 more source

Assessment of Extramammary Paget Disease by Two-Photon Microscopy

Frontiers in Medicine, 2022
Two-photon microscopy techniques are non-linear optical imaging methods which are gaining momentum in the investigation of fixed tissue sections, fresh tissue or even for in vivo experiments. Two-photon excited fluorescence and second harmonic generation
Radu Hristu, Lucian G. Eftimie, Lucian G. Eftimie, Stefan G. Stanciu, Remus R. Glogojeanu, Pavel Gheorghita, Pavel Gheorghita, George A. Stanciu   +7 more
doaj   +1 more source

Label-free imaging immune cells and collagen in atherosclerosis with two-photon and second harmonic generation microscopy [PDF]

Journal of Innovative Optical Health Sciences, 2016
Atherosclerosis has been recognized as a chronic inflammation disease, in which many types of cells participate in this process, including lymphocytes, macrophages, dendritic cells (DCs), mast cells, vascular smooth muscle cells (SMCs).
Chunqiang Li, Riikka K. Pastila, Charles P. Lin   +2 more
doaj   +1 more source

Fluorescence radial fluctuation enables two-photon super-resolution microscopy

Frontiers in Cellular Neuroscience, 2023
Despite recent improvements in microscopy, it is still difficult to apply super-resolution microscopy for deep imaging due to the deterioration of light convergence properties in thick specimens.
Motosuke Tsutsumi, Motosuke Tsutsumi, Taiga Takahashi, Taiga Takahashi, Kentaro Kobayashi, Tomomi Nemoto, Tomomi Nemoto, Tomomi Nemoto   +7 more
doaj   +1 more source

Line-Shaped Illumination: A Promising Configuration for a Flexible Two-Photon Microscopy Setup

Applied Sciences, 2022
An innovative two-photon microscope exploiting a line-shaped illumination has been recently devised and then implemented. Such configuration allows to carry out a real-time detection by means of standard CCD cameras and is able to maintain the same ...
Jacopo Parravicini, Elton Hasani, Luca Tartara   +2 more
doaj   +1 more source

In vivo imaging with a water immersion objective affects brain temperature, blood flow and oxygenation

eLife, 2019
Previously, we reported the first oxygen partial pressure (Po2) measurements in the brain of awake mice, by performing two-photon phosphorescence lifetime microscopy at micrometer resolution (Lyons et al., 2016).
Morgane Roche, Emmanuelle Chaigneau, Ravi L Rungta, Davide Boido, Bruno Weber, Serge Charpak   +5 more
doaj   +1 more source

A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope

Journal of Innovative Optical Health Sciences, 2023
The volumetric imaging of two-photon microscopy expands the focal depth and improves the throughput, which has unparalleled superiority for three-dimension samples, especially in neuroscience.
Ting Mo, Yiran Liu, Fatao Bie, Zimin Dai, Jin Chang, Hui Gong, Wei Zhou   +6 more
doaj   +1 more source

Evaluating performance in three-dimensional fluorescence microscopy [PDF]

, 2007
In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including ...
JOHN M. MURRAY, Appleton, Paul L., Waters, Jennifer C., Murray, John M., Swedlow, Jason R.; id_orcid, JENNIFER C. WATERS, PAUL L. APPLETON, JASON R. SWEDLOW   +7 more
core   +1 more source