Needle beam two-photon microscopy for simultaneous multiplane neural and vascular imaging in awake mice [PDF]
PhotoniXComprehensive understanding of brain functions necessitates high-speed imaging of neuronal and vascular dynamics across extensive volumes. Functional neuroimaging investigations with two-photon microscopy are commonly hindered by its limited depth of ...
Quanyu Zhou +11 more
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Insight into pericytes in glioblastoma angiogenesis: In vivo tracking by two‐photon microscopy and proteomic profiling [PDF]
Animal Models and Experimental MedicineBackground Glioblastoma (GBM) is a highly aggressive brain tumor characterized by aberrant angiogenesis and an immunosuppressive microenvironment. Pericytes are aberrantly recruited but their spatiotemporal roles and molecular changes remain unclear ...
Qinghong Wang +5 more
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Two-Photon Laser Scanning Microscopy
, 2008 Since its inception more than 15 years ago, two-photon laser scanning microscopy (2PLSM) has found widespread use in biological and medical research. Two-photon microscopy is based on simultaneous absorption of two photons by fluorophores and subsequent ...
Nimmerjahn, A, Theer, P, Helmchen, F
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A new phase for two-photon microscopy [PDF]
Frontiers in Neuroscience, 2009 Two-photon excitation has enabled remarkable advances in our understanding of the nervous system. Its three-dimensional resolution, superior depth penetration, and minimal phototoxicity for out-of-focus portions of the sample have made it the technique of choice for an impressive array of applications.
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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre [PDF]
, 2004 We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques.
McConnell, G., Riis, E.
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Two-Photon Imaging to Unravel the Pathomechanisms Associated with Epileptic Seizures: A Review
Applied Sciences, 2021 Despite extensive research, the exact pathomechanisms associated with epileptic seizure formation and propagation have not been elucidated completely. Two-photon imaging (2PI) is a fluorescence-based microscopy technique that, over the years, has been ...
Luqman Khan +7 more
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Adaptive Optics in Two-Photon Microscopy and Two-Photon Lithography
, 2018 Two-photon microscopy has become an indispensable tool in biological research allowing imaging of intracellular structures especially deep in tissue. Due to its capability of three-dimensional (3D) fabrication in lithography, two-photon excitation is also an essential tool for nanofabrication.
Neuwirth, Julia
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Comparing confocal and two-photon Ca2+ imaging of thin low-scattering preparations
Biophysical Reports, 2023 Ca2+ imaging provides insight into biological processes ranging from subcellular dynamics to neural network activity. Two-photon microscopy has assumed a dominant role in Ca2+ imaging.
Jinbo Cheng +3 more
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ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION [PDF]
Journal of Innovative Optical Health Sciences, 2013 Understanding brain structure and function, and the complex relationships between them, is one of the grand challenges of contemporary sciences. Thanks to their flexibility, optical techniques could be the key to explore this complex network.
L. SILVESTRI +4 more
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Label-free imaging immune cells and collagen in atherosclerosis with two-photon and second harmonic generation microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2016 Atherosclerosis has been recognized as a chronic inflammation disease, in which many types of cells participate in this process, including lymphocytes, macrophages, dendritic cells (DCs), mast cells, vascular smooth muscle cells (SMCs).
Chunqiang Li +2 more
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