Results 31 to 40 of about 20,254,963 (292)
Study of dendritic spine compartmentalization : a correlative fluorescence light microscopy-electron microscopy approach [PDF]
, 2014 Neurons communicate with each other through synapses. Most excitatory synapses contact small protrusions called dendritic spines. Spines are connected to dendrites by a very thin stalk called the “spine neck” which restricts diffusion between the spine ...
Cyprien, Vivien
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Imaging microglia surveillance during sleep-wake cycles in freely behaving mice
eLife, 2023 Microglia surveillance manifests itself as dynamic changes in cell morphology and functional remodeling. Whether and how microglia surveillance is coupled to brain state switches during natural sleep-wake cycles remains unclear. To address this question,
Xiaochun Gu +11 more
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Encoded multisite two-photon microscopy [PDF]
Proceedings of the National Academy of Sciences, 2013 The advent of scanning two-photon microscopy (2PM) has created a fertile new avenue for noninvasive investigation of brain activity in depth. One principal weakness of this method, however, lies with the limit of scanning speed, which makes optical interrogation of action potential-like activity in a neuronal network problematic.
Mathieu, Ducros +4 more
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Photobleaching in Two-Photon Excitation Microscopy [PDF]
Biophysical Journal, 2000 The intensity-squared dependence of two-photon excitation in laser scanning microscopy restricts excitation to the focal plane and leads to decreased photobleaching in thick samples. However, the high photon flux used in these experiments can potentially lead to higher-order photon interactions within the focal volume.
Patterson, George H., Piston, David W.
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Improving Focal Photostimulation of Cortical Neurons with Pre-derived Wavefront Correction
Frontiers in Cellular Neuroscience, 2017 Recent progress in neuroscience to image and investigate brain function has been made possible by impressive developments in optogenetic and opto-molecular tools.
Vincent R. Daria +6 more
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Two-photon microscopy in pulmonary research [PDF]
Seminars in Immunopathology, 2010 As the lung is constantly exposed to both innocuous and potentially noxious antigens, a thorough understanding of both innate and adaptive immune responses in this organ is of the essence. Imaging modalities such as magnetic resonance imaging, positron emission tomography, and confocal microscopy have expanded our knowledge about various molecular ...
Ruben G, Nava +5 more
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Evaluating performance in three-dimensional fluorescence microscopy [PDF]
, 2007 In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including ...
JOHN M. MURRAY +7 more
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Four-dimensional multi-site photolysis of caged neurotransmitters
Frontiers in Cellular Neuroscience, 2013 Neurons receive thousands of synaptic inputs that are distributed in space and time. The systematic study of how neurons process these inputs requires a technique to stimulate multiple yet highly targeted points of interest along the neuron's ...
Mary Ann eGo +7 more
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Multi-Color Two-Photon Microscopic Imaging Based on a Single-Wavelength Excitation
Biosensors, 2022 Two-photon probes with broad absorption spectra are beneficial for multi-color two-photon microscopy imaging, which is one of the most powerful tools to study the dynamic processes of living cells.
Wei Yan +6 more
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Epifluorescence collection in two-photon microscopy
Applied Optics, 2002 We present a simple model to describe epifluorescence collection in two-photon microscopy when one images in a turbid slab with an objective. Bulk and surface scattering determine the spatial and angular distributions of the outgoing fluorescence photons at the slab surface, and geometrical optics determines how efficiently the photons are collected ...
Beaurepaire, Emmanuel, Mertz, Jérôme
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