Results 141 to 150 of about 2,798,160 (306)
Mesoscopic calcium imaging in a head-unrestrained male non-human primate using a lensless microscope
Nature CommunicationsMesoscopic calcium imaging enables studies of cell-type specific neural activity over large areas. A growing body of literature suggests that neural activity can be different when animals are free to move compared to when they are restrained ...
Jimin Wu +4 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The development of a high speed 3D 2-photon microscope for neuroscience [PDF]
, 2010 The progress of neuroscience is limited by the instrumentation available to it for studying the brain. At present, there is a serious instrumentation gap between functional Magnetic Resonance Imaging (fMRI) of whole brains and the microscopic scale ...
Kirkby, P.A.
core
Three-plane calcium imaging of Zebrafish brain in vivo.MP4
, 2019 Three-plane calcium imaging of Zebrafish brain in ...
Lingjie Kong (2284588)
core +1 more source
eLifeDespite substantial progress in mapping the trajectory of network plasticity resulting from focal ischemic stroke, the extent and nature of changes in neuronal excitability and activity within the peri-infarct cortex of mice remains poorly defined.
Mischa Vance Bandet, Ian Robert Winship
doaj +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
A neural correlate of individual odor preference in Drosophila
eLifeBehavior varies even among genetically identical animals raised in the same environment. However, little is known about the circuit or anatomical origins of this individuality.
Matthew A Churgin +5 more
doaj +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Analysis pipelines for calcium imaging data
Current Opinion in Neurobiology, 2019 Calcium imaging is a popular tool among neuroscientists because of its capability to monitor in vivo large neural populations across weeks with single neuron and single spike resolution. Before any downstream analysis, the data needs to be pre-processed to extract the location and activity of the neurons and processes in the observed field of view. The
openaire +2 more sources
Frontiers in BiophysicsCalcium not only contributes to changes in membrane potential but also acts as a central regulator of multiple cellular processes. Invertebrates have had a critical role throughout history as biological models for studying the nervous system at the ...
María Laura Ríos-Reyes +3 more
doaj +1 more source