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Fluorescence lifetime imaging of intracellular calcium
Journal of Fluorescence, 1993Fluorescence lifetime imaging microscopy (FLIM) is a new methodology for studying the spatial and temporal dynamics of macromolecule, molecules, and ions in living cells. In FLIM image contrast is derived from the mean fluorescence lifetime at each point in a two-dimensional image.
H, Szmacinski +4 more
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Hybrid Imaging: Calcium Score and Myocardial Perfusion Imaging
Seminars in Nuclear MedicineCoronary heart disease (CHD) remains the top cause of death due to cardiovascular conditions worldwide, with someone suffering a myocardial infarction every 40 seconds. This highlights the importance of non-invasive imaging technologies like myocardial perfusion imaging (MPI), which are crucial for detecting coronary artery disease (CAD) early, even ...
Malek Nayfeh +5 more
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Coronary artery calcium imaging
2022Abstract Coronary artery calcium correlates with the extent of coronary atherosclerosis and has been shown to be a strong and independent predictor of long-term cardiovascular. Coronary artery calcium estimates can be obtained from a non-contrast CT scan at low radiation exposure.
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1999
Confocal fluorescence microscopy will, in principle, collect light emitted by only that dye that lies in the plane of focus. Signal from dye in regions of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca++]i) use monolayers of cells in culture. After loading a calcium indicator dye
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Confocal fluorescence microscopy will, in principle, collect light emitted by only that dye that lies in the plane of focus. Signal from dye in regions of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca++]i) use monolayers of cells in culture. After loading a calcium indicator dye
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Calcium Imaging: A Primer for Mycologists
Fungal Genetics and Biology, 1998This article aims to encourage more fungal biologists to consider the imaging of cytoplasmic Ca2+ fluxes. Compared to other organisms, for fungi there have been remarkably few attempts to characterize the role of Ca2+ fluxes in signal transduction and general cellular activities, even though other approaches indicate that fungal growth and development ...
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Image Analysis of Calcium Release Dynamics
2008Many aspects of animal development are dependent on the dynamic release of calcium (Ca2+) ions. Although Ca2+ release within a cell is tightly controlled, how the release dynamics result in a specific biological output in embryonic development is less clear.
Christina M, Freisinger +2 more
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Imaging approaches to measuring lysosomal calcium
2015Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca(2+) signals. The role of Ca(2+) in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca(2+) stores; they actively sequester Ca(2+) in their lumina and release it to the ...
Anthony J, Morgan +2 more
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Coronary Artery Calcium Imaging
2010Coronary artery calcium imaging (CAC) in the asymptomatic population has been investigated in tens of thousands of primary prevention patients. The consistent results in both retrospective and prospective studies have confirmed the vast superiority of CAC to all risk-factor based predictive paradigms, e.g., Framingham Risk Score.
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Cellular calcium imaging: so, what’s new?
Trends in Cell Biology, 2000The ability to uncover the fine details of intracellular Ca(2+) signals has improved remarkably in recent years, largely as a result of developments in methods for reporting Ca(2+), coupled with great improvements in measurement instrumentation. The cell biologist wishing to image intracellular Ca(2+) has a range of options to consider.
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