Development of a High-Throughput Calcium Mobilization Assay for CCR6 Receptor Coupled to Hydrolase Activity Readout [PDF]
Biomedicines, 2022 CCR6 is a chemokine receptor highly implicated in inflammatory diseases and could be a potential therapeutic target; however, no therapeutic agents targeting CCR6 have progressed into clinical evaluation.
Sara Gómez-Melero +7 more
doaj +4 more sources
Kynurenic acid ameliorates NLRP3 inflammasome activation by blocking calcium mobilization via GPR35 [PDF]
Frontiers in Immunology, 2022 The inflammasome has been linked to diverse inflammatory and metabolic diseases, and tight control of inflammasome activation is necessary to avoid excessive inflammation. Kynurenic acid (KA) is a tryptophan metabolite in the kynurenine pathway. However,
Tianyin Sun +10 more
doaj +2 more sources
Curcumin Inhibits Protease Activated Receptor 2-Induced ERK Phosphorylation Calcium Mobilization and Anti-Apoptotic Signaling in Inflammation-Driven Colorectal Cancer Cells [PDF]
CellsBackground: Chronic inflammation drives colorectal cancer (CRC) progression, with PAR-2, a G-protein coupled receptor, linking extracellular inflammatory signals to tumor-promoting pathways via ERK1/2 phosphorylation, calcium mobilization, TNF-α ...
Rajashree Patnaik +4 more
doaj +2 more sources
Calcium Mobilization in Endothelial Cell Functions. [PDF]
Int J Mol Sci, 2019 Endothelial cells (ECs) constitute the innermost layer that lines all blood vessels from the larger arteries and veins to the smallest capillaries, including the lymphatic vessels. Despite the histological classification of endothelium of a simple epithelium and its homogeneous morphological appearance throughout the vascular system, ECs, instead, are ...
Filippini A, D'Amore A, D'Alessio A.
europepmc +4 more sources
Molecules, 2012 Novel cADPR mimics, which integrate nucleobase, northern and southern ribose modifications were synthesized. The key steps of the synthesis were a Cu(I)-catalyzed Hüisgen [3+2] cycloaddition and a microwave-assisted intramolecular pyrophosphorylation ...
Liangren Zhang +6 more
doaj +3 more sources
Development and validation of a high-throughput calcium mobilization assay for the orphan receptor GPR88 [PDF]
Journal of Biomedical Science, 2017 Background GPR88 is an orphan G protein-coupled receptor highly expressed in the striatum and is implicated in basal ganglia-associated disorders. However, the receptor functions of GPR88 are still largely unknown due to the lack of potent and selective ...
Ann M. Decker +6 more
doaj +2 more sources
Dopamine internalization via Uptake2 and stimulation of intracellular D5-receptor-dependent calcium mobilization and CDP-diacylglycerol signaling [PDF]
Frontiers in PharmacologyDopamine stimulates CDP-diacylglycerol biosynthesis through D1-like receptors, particularly the D5 subtype most of which is intracellularly localized.
Wenfei Kang +7 more
doaj +2 more sources
Neuroendocrine control of calcium mobilization in the fruit fly. [PDF]
NatureCalcium (Ca2+) is an essential mineral that must be strictly regulated to support numerous physiological activities1,2. Extracellular fluid Ca2+ is regulated in vertebrates through endocrine systems that manage the vast Ca2+ reservoir in the bones3-6, but the Ca2+ regulatory mechanisms used by invertebrates, which lack bones, remain largely unclear ...
Okamoto N +4 more
europepmc +3 more sources
Small Extracellular Vesicles with a High Sphingomyelin Content Isolated from Hypertensive Diabetic db/db Mice Inhibits Calcium Mobilization and Augments Amiloride-Sensitive Epithelial Sodium Channel Activity [PDF]
BiologyExtracellular vesicles (EVs) contain bioactive lipids that play a key role in pathophysiology. We hypothesized that EVs released from salt-loaded hypertensive diabetic db/db mice have increased bioactive lipid content that inhibits intracellular calcium ...
Hunter Ramsay +3 more
doaj +2 more sources
Evaluation of No-Wash Calcium Assay Kits: Enabling Tools for Calcium Mobilization
Journal of Biomolecular Screening, 2007 The no-wash calcium assay kits developed by Molecular Devices Corporation have greatly enhanced the throughput of cell-based calcium mobilization high-throughput screening (HTS) assays and enabled screening using nonadherent cells. The fluorescent imaging plate reader (FLIPR) Calcium 3 Assay Kit, optimal for targets that have proteins or peptides as ...
Lisa K Minor, Yuanping Wang
exaly +3 more sources