Results 1 to 10 of about 470 (114)
The kinetics of calcium binding to fura-2 and indo-1 [PDF]
FEBS Letters, 1987 The kinetics of Ca2+ dissociation from fura‐2 and indo‐1 were measured using a stopped‐flow spectrofluorimeter. The dissociation rate constants were 84 s−1 and 130 s−1, respectively, in 0.1 M KCl at 20°C. The rate constants were insensitive to pH over the range 7.0 to 8.0.
Clive R Bagshaw, C C Ashley
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The use of fura-2 for estimating ca buffers and ca fluxes
Neuropharmacology, 1995 The compound fura-2 (Grynkiewicz et al., J. Biol. Chem. 260, 3440-3450, 1985) is generally known as an indicator dye for measuring the concentration of free calcium ([Ca2+]) inside living cells. It should be appreciated, however, that this is not what it actually is.
Erwin Neher
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Leakage of the Fluorescent Ca2+ Indicator Fura-2 in Smooth Muscle
The Japanese Journal of Pharmacology, 1993 The movement of a fluorescent intracellular Ca2+ indicator, fura-2, in smooth muscle was examined. Strips of rat and rabbit aortas and bovine trachea were loaded with the acetoxymethyl ester of fura-2 (fura-2/AM), followed by washing with normal physiological solution. Not only fura-2/AM but also fura-2 was detected in the washout solution.
Masaomi Tajimi, Asaki Abe, H Karaki
exaly +4 more sources
Scientific Reports, 2021 Supplementation of cancer cells exposed to 5-fluorouracil (FUra) and folinic acid (FA) with high concentration pyridoxal 5′-phosphate, the cofactor of vitamin B6, potentiates the cytotoxicity of FUra in a synergistic interaction mode.
David Machover +7 more
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Scientific Reports, 2022 High concentration pyridoxal 5’-phosphate, the cofactor of vitamin B6, potentiates cytotoxicity in cancer cells exposed to 5-fluorouracil (FUra) and folinic acid (FA).
David Machover +8 more
doaj +1 more source
Mepivacaine-induced intracellular calcium increase appears to be mediated primarily by calcium influx in rat aorta without endothelium [PDF]
Korean Journal of Anesthesiology, 2014 BackgroundMepivacaine induces contraction or decreased blood flow both in vivo and in vitro. Vasoconstriction is associated with an increase in the intracellular calcium concentration ([Ca2+]i).
Seong-Ho Ok +4 more
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Cellular fura‐2 Manganese Extraction Assay (CFMEA) [PDF]
Current Protocols in Toxicology, 2011 AbstractCellular manganese (Mn) uptake and transport dynamics can be measured using a cellular fura‐2 manganese extraction assay (CFMEA). The assay described here uses immortalized murine striatal cell line and primary cortical astrocytes, but the method is equally adaptable to other cultured mammalian cells.
Gunnar F, Kwakye +3 more
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Resveratrol Interferes with Fura-2 Intracellular Calcium Measurements [PDF]
Journal of Fluorescence, 2013 Resveratrol, a naturally occurring polyphenol found in some fruits and especially in grapes, has been reported to provide diverse health benefits. Resveratrol's mechanism of action is the subject of many investigations, and some studies using the ratiometric calcium indicator Fura-2 suggest that it modulates cellular calcium responses.
Richard F, Kopp +2 more
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Chinese Journal of Physiology, 2020 Thioridazine, belonging to first-generation antipsychotic drugs, is a prescription used to treat schizophrenia. However, the effect of thioridazine on intracellular Ca2+ concentration ([Ca2+]i) and viability in human liver cancer cells is unclear.
I-Shu Chen +6 more
doaj +1 more source
Bio-Protocol, 2021 Calcium signaling is an emerging mechanism by which bacteria respond to environmental cues. To measure the intracellular free-calcium concentration in bacterial cells, [Ca2+]i, a simple spectrofluorometric method based on the chemical probe Fura 2 ...
Simone Trabalza +5 more
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