Results 41 to 50 of about 24,447,932 (192)
In this paper, we design, fabricate, and test a PVAc-based benzophenone-8 (PVAcB-8) emission filter to monitor calcium changes in live neurons. We loaded neurons with fura-2 AM, a calcium-sensitive fluorescent dye, which has dual excitation wavelengths ...
Lior Blockstein +4 more
doaj +1 more source
Folinic Acid Enhanced Cytotoxicity of Fluoropyrimidines In Human Breast Cancer Cells
This report describes the effects of the natural diastereoisomer of 5-formyltetrahydrofolate (6S)- and of the mixture of both the natural and unnatural diastereoisomers (6RS)-5-HCO-H4PteGlu (folinate) on the cytotoxicity of 5-fluoro-2′-deoxyuridine ...
Mandelbaum-Shavit Frederika
doaj +1 more source
Fine structural detection of calcium ions by photoconversion
We propose a tool for a rapid high-resolution detection of calcium ions which can be used in parallel with other techniques. We have applied a new approach by photo-oxidation of diaminobenzidine in presence of the emission of an excited fluorochrome ...
V. Poletto +5 more
doaj +1 more source
5-Fluorouracil (5FUra) is the third most popular chemotherapeutic component employed to treat solid tumors. In the present study, we aimed to appraise the silymarin (SM) and silymarin nanoemulsion (SMN) effect on 5FUra-induced gastrointestinal toxicity ...
Soheila Safarpour +5 more
doaj +1 more source
2-oxoglutarate modulates the affinity of FurA for the ntcA promoter in Anabaena sp. PCC 7120 [PDF]
2-oxoglutarate (2-OG) is a central metabolite that acts as a signaling molecule informing about the status of the carbon/nitrogen balance of the cell. In recent years, some transcriptional regulators and even two-component systems have been described as ...
María Teresa Bes +13 more
core +1 more source
Background Calcitonin gene-related peptide (CGRP) is one of the most potent endogenous vasodilators identified to date. The present study elucidates the differential interaction of CGRP, its receptor and the effect of the CGRP-receptor antagonist ...
André Erdling +2 more
doaj +1 more source
The interaction of Fura-2 with Ca2+ is studied using steady fluorescence technique.The effect of pH on the spectra behavior of Fura-2 in the presence of Ca2+ is investigated,the excitation maxima(340 nm)and the isobestic point(360 nm)for the fluorescence
Shao-min SHUANG1 +6 more
doaj
Cytosolic Ca2+ measurements by ratiometric fluorescence microscopy in melanoma cells
Summary: Cytosolic Ca2+ levels are maintained at low nanomolar concentrations, and disruption of Ca2+ homeostasis is associated with cell/tissue damage.
Tiago Rodrigues, Letícia Silva Ferraz
doaj +1 more source
Changes of cytosolic Ca2+ interfere with measurements of cytosolic Mg2+ using mag-fura-2
In rat pancreatic and submandibular gland acini during exposure to carbachol, changes in the fluorescence emission intensity ratio (R) of acini loaded with mag-fura-2 resemble changes in cytosolic Ca2+ concentration (Ca2+i) in acini loaded with fura-2 ...
M. P. Ryan, R. W. Brinck, T. W. Hurley
core +1 more source
Fura‐2 calcium transients in frog skeletal muscle fibres.
1. Intact single twitch fibres from frog muscle were mounted at long sarcomere spacing (3.5‐4.2 microns) on an optical bench apparatus for the measurement of absorbance and fluorescence signals following the myoplasmic injection of either or both of the Ca2+ indicator dyes Fura‐2 and Antipyrylazo III.
S M, Baylor, S, Hollingworth
openaire +3 more sources

