Results 41 to 50 of about 24,447,932 (192)

A PVAc-Based Benzophenone-8 Filter as an Alternative to Commercially Available Dichroic Filters for Monitoring Calcium Activity in Live Neurons via Fura-2 AM

open access: yesIEEE Photonics Journal, 2012
In this paper, we design, fabricate, and test a PVAc-based benzophenone-8 (PVAcB-8) emission filter to monitor calcium changes in live neurons. We loaded neurons with fura-2 AM, a calcium-sensitive fluorescent dye, which has dual excitation wavelengths ...
Lior Blockstein   +4 more
doaj   +1 more source

Folinic Acid Enhanced Cytotoxicity of Fluoropyrimidines In Human Breast Cancer Cells

open access: yesPteridines, 1993
This report describes the effects of the natural diastereoisomer of 5-formyltetrahydrofolate (6S)- and of the mixture of both the natural and unnatural diastereoisomers (6RS)-5-HCO-H4PteGlu (folinate) on the cytotoxicity of 5-fluoro-2′-deoxyuridine ...
Mandelbaum-Shavit Frederika
doaj   +1 more source

Fine structural detection of calcium ions by photoconversion

open access: yesEuropean Journal of Histochemistry, 2016
We propose a tool for a rapid high-resolution detection of calcium ions which can be used in parallel with other techniques. We have applied a new approach by  photo-oxidation of diaminobenzidine in presence of the emission of an excited fluorochrome ...
V. Poletto   +5 more
doaj   +1 more source

The protective effects of silymarin nanoemulsion on 5-fluorouracil-induced gastrointestinal toxicity in rats

open access: yesSaudi Pharmaceutical Journal, 2023
5-Fluorouracil (5FUra) is the third most popular chemotherapeutic component employed to treat solid tumors. In the present study, we aimed to appraise the silymarin (SM) and silymarin nanoemulsion (SMN) effect on 5FUra-induced gastrointestinal toxicity ...
Soheila Safarpour   +5 more
doaj   +1 more source

2-oxoglutarate modulates the affinity of FurA for the ntcA promoter in Anabaena sp. PCC 7120 [PDF]

open access: yes, 2019
2-oxoglutarate (2-OG) is a central metabolite that acts as a signaling molecule informing about the status of the carbon/nitrogen balance of the cell. In recent years, some transcriptional regulators and even two-component systems have been described as ...
María Teresa Bes   +13 more
core   +1 more source

Differential inhibitory response to telcagepant on αCGRP induced vasorelaxation and intracellular Ca2+ levels in the perfused and non-perfused isolated rat middle cerebral artery

open access: yesThe Journal of Headache and Pain, 2017
Background Calcitonin gene-related peptide (CGRP) is one of the most potent endogenous vasodilators identified to date. The present study elucidates the differential interaction of CGRP, its receptor and the effect of the CGRP-receptor antagonist ...
André Erdling   +2 more
doaj   +1 more source

Analytical Behavior of Fura-2 and Its Determination of [Ca2+]i in Lymphocytes Treated with Cefotaxime

open access: yesJournal of Measurement Science and Instrumentation, 2010
The interaction of Fura-2 with Ca2+ is studied using steady fluorescence technique.The effect of pH on the spectra behavior of Fura-2 in the presence of Ca2+ is investigated,the excitation maxima(340 nm)and the isobestic point(360 nm)for the fluorescence
Shao-min SHUANG1   +6 more
doaj  

Cytosolic Ca2+ measurements by ratiometric fluorescence microscopy in melanoma cells

open access: yesSTAR Protocols, 2021
Summary: Cytosolic Ca2+ levels are maintained at low nanomolar concentrations, and disruption of Ca2+ homeostasis is associated with cell/tissue damage.
Tiago Rodrigues, Letícia Silva Ferraz
doaj   +1 more source

Changes of cytosolic Ca2+ interfere with measurements of cytosolic Mg2+ using mag-fura-2

open access: yes, 1992
In rat pancreatic and submandibular gland acini during exposure to carbachol, changes in the fluorescence emission intensity ratio (R) of acini loaded with mag-fura-2 resemble changes in cytosolic Ca2+ concentration (Ca2+i) in acini loaded with fura-2 ...
M. P. Ryan, R. W. Brinck, T. W. Hurley
core   +1 more source

Fura‐2 calcium transients in frog skeletal muscle fibres.

open access: yesThe Journal of Physiology, 1988
1. Intact single twitch fibres from frog muscle were mounted at long sarcomere spacing (3.5‐4.2 microns) on an optical bench apparatus for the measurement of absorbance and fluorescence signals following the myoplasmic injection of either or both of the Ca2+ indicator dyes Fura‐2 and Antipyrylazo III.
S M, Baylor, S, Hollingworth
openaire   +3 more sources

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