Ca2+ binding kinetics of fura-2 and azo-1 from temperature-jump relaxation measurements [PDF]
, 1988 The Ca2+-binding kinetics of fura-2 and azo-1 were studied using temperature-jump relaxation methods. In 140 mM KCl at 20 degrees C, the association and dissociation rate constants for fura-2 were 6.02 x 10(8) M-1s-1 and 96.7 s-1, respectively.
Kao, J.P., Tsien, R.Y.
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Action of econazole on Ca2+ levels and cytotoxicity in OC2 human oral cancer cells
Journal of Dental Sciences, 2023 Background/purpose: Econazole is an antifungal drug. Antifungal activity of econazole against non-dermatophyte molds was reported. Econazole inhibited Ca2+ channels and stimulated cytotoxicity in lymphoma and leukemia cells.
Jue-Long Wang +2 more
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Glucose-stimulated efflux of fura-2 in pancreatic β-cells is prevented by probenecid
, 1989 Fura-2 loaded pancreatic β-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca 2+ concentration ([Ca 2+] i), insulin release and efflux of indicator.
Per Arkhammar +5 more
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Free calcium concentrations in bullfrog rods determined in the presence of multiple forms of Fura-2 [PDF]
, 1994 We employed the fluorescent calcium indicator Fura-2, loaded into intact retinas of the bullfrog Rana catesbeiana, to measure free calcium concentrations in the rod outer-segment cytosol.
Younger, J.P. +2 more
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Thymol-evoked Ca Mobilization and Ion Currents in Pituitary GH Cells
Natural Product Communications, 2009 In this study, an attempt was made to elucidate the effects of thymol, a monocyclic phenolic compound, on Ca 2+ mobilization and ion currents in pituitary GH 3 cells with the aid of fura-2 fluorimetry and the whole-cell voltage-clamp technique.
Ai-Yu Shen +5 more
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Endogenous heavy metal ions perturb fura-2 measurements of basal and hormone-evoked Ca2+ signals [PDF]
, 1996 Using the membrane-permeant chelator of heavy metal ions, N,N,N',N'-tetrakis(2-pyridylmethyl)ethylene diamine (TPEN), we demonstrate that in pancreatic acinar cells, hepatocytes, and a variety of mammalian cell lines, endogenous heavy metal ions bind to ...
Snitsarev, V.A. +5 more
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Peripheral nerve injury increases glutamate-evoked calcium mobilization in adult spinal cord neurons
Molecular Pain, 2012 Background Central sensitization in the spinal cord requires glutamate receptor activation and intracellular Ca2+ mobilization. We used Fura-2 AM bulk loading of mouse slices together with wide-field Ca2+ imaging to measure glutamate-evoked increases in ...
Doolen Suzanne +3 more
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Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2 [PDF]
, 1990 We studied intracellular binding and possible compartmentalization of the fluorescent Ca2+ indicators, indo-1 and fura-2, in single mammalian cardiac ventricular cells that had been loaded with indo-1 and fura-2 by exposure to the acetoxymethylester form
Blatter, L.A., Wier, W.G.
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Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2 [PDF]
, 1993 A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV).
Alexander Burger +9 more
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Molecular and functional characterization of voltage-gated sodium channels in human sperm
Reproductive Biology and Endocrinology, 2009 Background We have investigated the expression of voltage-gated sodium channels in human spermatozoa and characterized their role in sperm motility. Methods Freshly ejaculated semen was collected from thirty normozoospermic human donors, with each donor ...
Cejudo-Román Antonio +5 more
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