Results 71 to 80 of about 24,447,932 (192)
Conversion of esterified fura-2 and indo-1 to Ca2+-sensitive forms by mitochondria
Rat liver mitochondria are shown to convert the acetoxymethyl ester forms of fura-2 and indo-1 into Ca2+-dependent forms of these indicators. The excitation spectrum of the Ca2+-dependent conversion product of fura-2 acetoxymethyl ester is shown to be ...
D. Restrepo, K. K. Gunter, T. E. Gunter
core +1 more source
Background A higher increase in intracellular Na+ via Na+/H+ exchanger (NHE) during ischemia has been reported in type 2 diabetic mouse hearts.
Anzawa Ryuko +5 more
doaj +1 more source
Spectroscopic Properties of Fluorescence Dye Fura-2 with Various Divalent Cations.
We examined the effects of divalent cations on the fluorescence excitation spectra of fura-2 from a 2 ml solution containing 1 microM fura-2 with an excitation wavelength of 300-425 nm and an emission wavelength of 500 nm. The fluorescence spectra in solutions of fura-2-Ca2+, fura-2-Ba2+, and fura-2-Sr2+ complexes show almost the same pattern.
HATAE, Junna +2 more
openaire +3 more sources
H2O2 treatment increases Fura-2 fluorescence and cardiac myocyte contractility.
Panel A shows the effects of hydrogen peroxide (H2O2, 25 µM) on F340/F380 ratio in Fura-2 loaded adult mouse cardiac myocytes. Cells were loaded with Fura-2 AM (1 µM) for 20 minutes prior to microscopic analysis.
Juliano L. Sartoretto (138024) +6 more
core +1 more source
Inhibitors of membrane transport system for organic anions block fura-2 excretion from PC12 and N2A cells [PDF]
The neuroblastoma-like cell line N2A and the pheochromocytoma-like cell line PC12 excrete about 20-25% of the intracellular fluorescent Ca2+ indicator fura-2 during 10 min of incubation at 37 degrees C.
DI VIRGILIO, Francesco +2 more
core +1 more source
Mycobacterium tuberculosis FurA autoregulates its own expression
The furA-katG region of Mycobacterium tuberculosis, encoding a Fur-like protein and the catalase-peroxidase, is highly conserved among mycobacteria. Both genes are induced upon oxidative stress. In this work we analyzed the M.
C. Sala +6 more
core +2 more sources
目的 探讨谷氨酸 (Glutamicacid ,Glu)的神经性兴奋毒损伤作用机制。方法 在体外快速剥离新生大鼠 (0 1天 )双侧海马 ,制成海马神经细胞悬液 ,用Fura 2 /AM负载 ,建立了Fura 2 /AM检测海马神经细胞胞内游离钙离子浓度 ([Ca2 + ]i)的方法 ,并检测Glu兴奋毒对海马神经细胞 [Ca2 + ]i的影响。结果 Fura 2单细胞检测表明 ,静息状态下海马神经细胞 [Ca2 + ]i=2 5 2 .6± 36 .1(nmol/L) ,Glu可使海马神经细胞 ...
咸云淑, 熊文, 程刚
doaj
Background Lysophosphatidylcholine (LPC), an atherogenic component of oxidized low-density lipoprotein, has been shown to induce the attenuation of endothelium-dependent vascular relaxation. Although benidipine, a dihydropyridine-calcium channel blocker,
Wada Michihito +2 more
doaj +1 more source
Stimulated calcium efflux from fura‐2‐loaded human platelets.
1. The reduction of cytoplasmic free calcium, [Ca2+]i following stimulation, has been investigated in fura‐2‐loaded human platelets in the presence of low extracellular calcium concentration. Thrombin produced a rapid rise in [Ca2+]i which then fell back to the basal level within 2 min. 2.
T J, Rink, S O, Sage
openaire +3 more sources
Free cytosolic Ca2+ was measured with sub-micrometer-tip, double-barrelled, Ca(2+)-selective electrodes and fura 2 in rat mesenteric resistance arteries.
H. Nilsson +4 more
core +1 more source

