Results 81 to 90 of about 24,447,932 (192)

Determination of the Intracellular Calcium Concentration in Peritoneal Macrophages Using Microfluorimetry

open access: yesBio-Protocol, 2013
Calcium is one of the most important intracellular messengers in biological systems. Ca2+ microfluorimetry is a valuable tool to assess information about mechanisms involved in the regulation of intracellular Ca2+ levels in research on cells and in ...
Silvia González-Ramos   +5 more
doaj   +1 more source

Flow Cytometric Quantification of Glucose-Stimulated β-Cell Metabolic Flux Can Reveal Impaired Islet Functional Potency

open access: yesCell Transplantation, 2008
The objective of this study was to develop a multiparametric flow cytometry assay to simultaneously quantify isolated pancreatic islet cell viability, apoptosis, and glucose-induced metabolic flux.
Matthew S. Hanson   +4 more
doaj   +1 more source

Nanosecond fluorescence microscopy. Emission kinetics of fura-2 in single cells

open access: yesBiophysical Journal, 1991
A microscope based time-correlated single photon counting instrument has been constructed to measure fluorescence intensity and emission anisotropy decays from fluorophores in single cells on a nanosecond time scale. The sample is excited and the emission collected using epi-illumination optics with frequency-doubled pulses from the cavity-dumped ...
Keating, S.M., Wensel, T.G.
openaire   +2 more sources

Effect of DFP on loading of fura 2/AM and quin 2/AM into single smooth muscle cells prepared from guinea pig taenia coli [PDF]

open access: yes, 1987
The effect of diisopropyl fluorophosphate (DFP), a potent cholinesterase (ChE) inhibitor, on loading quin 2 acetoxymethyl ester (quin 2/AM) and fura 2/AM into smooth muscle cells isolated from guinea pig taenia coli was investigated ...
Hideto Oyamada   +9 more
core   +1 more source

Effects of benzo(a)pyrene exposure on the ATPase activity and calcium concentration in the hippocampus of neonatal rats

open access: yesInternational Journal of Occupational Medicine and Environmental Health, 2017
Objectives: To investigate whether postnatal benzo(a)pyrene (B(a)P) exposure caused the impairments on the process of neurodevelopment and the alteration in the calcium medium in the neonatal rats. Material and Methods: Eighty neonatal Sprague Dawley (SD)
Kai Yang   +4 more
doaj   +1 more source

Ratiometric analysis of Fura Red calcium dye and Fura Red titration.

open access: yes, 2015
Expanded human T cells were loaded with increasing concentrations of Fura Red, AM for 30 minutes. Intracellular calcium mobilization in response to ionomycin (5 μg/ml) was measured by flow cytometry. Arrows indicate the addition of ionomycin.
Satish Keshav (65388)   +3 more
core   +1 more source

Use of Fura 2 Fluorescent Dyes as Indicator for Studying Calcium Distribution in Several Plant Tissues

open access: yes, 2010
Calcium (Ca) is an essential plant nutrient and is important in determining quality of fruits in storage. The analytical method presently known to determine Ca concentration in vegetables measures total Ca, and there is a need to develop methods that can
Ruíz, Rafael [Chile. INIA La Platina]   +3 more
core   +1 more source

Instrumental and sensory textural properties of Fura

open access: yes, 2002
A uniaxial compression test and sensory textural analysis was conducted of fura samples made from millet flour. Significant differences (p < 0.05) existed among the samples for hardness (the force to compress the sample between molar teeth ...
Jideani, Victoria A
core   +1 more source

Resveratrol is not compatible with a Fura-2-based assay for measuring intracellular Ca(2+) signaling

open access: yes, 2014
Fura-2 is a commonly used fluorescent Ca2+ dye that allows an accurate determination of cytosolic Ca2+ levels by measuring the emission obtained at 510 nm after alternating excitation at 340 nm and 380 nm (F340/F380 ratio).
Paudel, Ram Chandra   +3 more
core   +1 more source

Home - About - Disclaimer - Privacy