Results 31 to 40 of about 24,447,932 (192)
, 2015 Intracellular calcium assay determined by fluorescent radiometric Ca2+ indicator Fura-2, in U87 CSCs treated with control, S, T, or S+T (* p value
Arun Dharmarajan (92865) +4 more
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PLoS ONE, 2012 The transfer of Ca(2+) from the cytosol into the lumen of mitochondria is a crucial process that impacts cell signaling in multiple ways. Cytosolic Ca(2+) ([Ca(2+)](cyto)) can be excellently quantified with the ratiometric Ca(2+) probe fura-2, while ...
Markus Waldeck-Weiermair +8 more
doaj +1 more source
Platelets, 2017 Previously, we have found that intracellular calcium homeostasis is altered in platelets from an experimental model of liver cirrhosis, the bile-duct ligated (BDL) rat; these alterations are compatible with the existence of a hypercoagulable state ...
Paola Romecín +6 more
doaj +1 more source
Resveratrol attenuates the Na(+)-dependent intracellular Ca(2+) overload by inhibiting H(2)O(2)-induced increase in late sodium current in ventricular myocytes. [PDF]
PLoS ONE, 2012 BACKGROUND/AIMS: Resveratrol has been demonstrated to be protective in the cardiovascular system. The aim of this study was to assess the effects of resveratrol on hydrogen peroxide (H(2)O(2))-induced increase in late sodium current (I(Na.L)) which ...
Chunping Qian +9 more
doaj +1 more source
Bio-Protocol, 2021 Elevations in cytosolic calcium (Ca2+) drive a wide array of immune cell functions, including cytokine production, gene expression, and cell motility. Live-cell imaging of cells loaded with ratiometric chemical Ca2+ indicators remains the gold standard ...
Amit Jairaman, Michael Cahalan
doaj +1 more source
Journal of Pharmacological Sciences, 2005 We have investigated whether the intracellular calcium concentration ([Ca2+]i) oscillations induced in astrocytes using the metabotropic glutamate-receptor agonist, (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) are Ca2+-dependent, using ...
Yoshitoku Yoshida +5 more
doaj +1 more source
Error correction due to background subtraction in ratiometric calcium measurements with CCD camera
Heliyon, 2020 Background: Ca2+ plays an important role in many physiological processes and an accurate study of these signals is important. In modern fluorescence microscopy, a charge-coupled device (CCD) camera is widely deployed for calcium imaging.
Vyacheslav M. Shkryl
doaj +1 more source
Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements [PDF]
, 1988 Binding of the fluorescent Ca2+ indicator dye fura-2 by intracellular constituents has been investigated by steady-state optical measurements. Fura-2's (a) fluorescence intensity, (b) fluorescence emission anisotropy, (c) fluorescence emission spectrum ...
Olson, A. +3 more
core +1 more source
Determination of spectral overlaps between fura-2 and cameleons.
, 2012 (A) Spectral overlaps between fura-2 and cameleons are demonstrated by plotting the Ca2+ induced changes of the fluorescence intensities at different excitation wavelength ranging from 315 nm –525 nm.
Markus Waldeck-Weiermair (133295) +8 more
core +1 more source
Intensity Modulation of Pseudocolor Images
BioTechniques, 1998 Fura-2 is a popular real-time fluorescent indicator used to image intracellular, free calcium concentrations ([Ca2+]i) in living cells. One difficulty with fura-2-based digital imaging is how to best display the [Ca2+]i information that is acquired ...
Lee E. Hinman, Paul J. Sammak
doaj +1 more source