Results 11 to 20 of about 24,447,932 (192)

Photophysics of the fluorescent Ca2+ indicator Fura-2 [PDF]

Biophysical Journal, 1995
The photophysics of the complex forming reaction of Ca2+ and Fura-2 are investigated using steady-state and time-resolved fluorescence measurements. The fluorescence decay traces were analyzed with global compartmental analysis yielding the following values for the rate constants at room temperature in aqueous solution with BAPTA as Ca2+ buffer: k01 ...
Van den Bergh, V., Boens, N., De Schryver, F.C., Ameloot, M., Steels, P., Gallay, J., Vincent, M., Kowalczyk, A.   +7 more
core   +5 more sources

Receptor-mediated Mn2+ influx in rat hepatocytes: comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt [PDF]

Biochemical Journal, 1994
In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl ...
G E, Kass, D L, Webb, S C, Chow, J, Llopis, P O, Berggren   +4 more
openaire   +3 more sources

Redox properties of the calcium chelator Fura-2 in mimetic biomembranes [PDF]

Cell Calcium, 2008
Fura-2 is one of the most commonly used fluorescent dyes to analyze the cytosolic Ca(2+) concentration ([Ca(2+)](i)) of living cells. Fura-2-dependent measurements of [Ca(2+)](i) are susceptible to changes of pH, reactive oxygen species concentration and membrane potential.
Gulaboski, Rubin, Pereira, Carlos M., Cordeiro, M Natália D S, Silva, A. Fernando, Hoth, Markus, Bogeski, Ivan   +5 more
openaire   +5 more sources

A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters [PDF]

Bio-Protocol
Manganese (Mn) is an essential trace element whose intracellular homeostasis is tightly controlled by specialized membrane transporters. Dysregulation of Mn transport leads to pathological Mn accumulation and severe human disease; however, efficient and ...
Huiwei Zhong, Xurui Shen, Hanting Yang
doaj   +2 more sources

Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader [PDF]

Bio-Protocol, 2017
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; Martínez et al.
Magdiel Martínez, Namyr Martínez, Walter Silva   +2 more
doaj   +2 more sources

Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2

BioMedical Engineering OnLine, 2012
Background We present an easily implementable method for measuring Fura-2 fluorescence from isolated mouse hearts using a commercially available switching light source and CCD camera. After calibration, it provides a good estimate of intracellular [Ca2+]
Venkataraman Raghav, Holcomb Mark R, Harder Rene, Knollmann Björn C, Baudenbacher Franz   +4 more
doaj   +2 more sources

Dynamic Ratiometric Imaging of Cytosolic Free Ca²⁺ in Skeletal Muscle Cells Using 340/385-nm Light-Emitting Diode Illuminators

IEEE Photonics Journal, 2018
Calcium-sensitive fluorescent indicators fall broadly into two categories, ratiometric (dual-wavelength) or single-wavelength indicators based on their response to a calcium elevation.
Manoop Chenchiliyan, Dana Adler Portal, Ruchira Chakraborty, Tal Shahar Ben-Gal, Assaf Deutsch, Eliahu Pewzner, Asher Shainberg, Hamootal Duadi, Dror Fixler   +8 more
doaj   +1 more source

High-Yield Method for Isolation and Culture of Endothelial Cells from Rat Coronary Blood Vessels Suitable for Analysis of Intracellular Calcium and Nitric Oxide Biosynthetic Pathways

Biological Procedures Online, 2002
We describe here a method for isolating endothelial cells from rat heart blood vessels by means of coronary microperfusion with collagenase. This methods makes it possible to obtain high amounts of endothelial cells in culture which retain the functional
Nistri Silvia, Mazzetti Luca, Failli Paola, Bani Daniele   +3 more
doaj   +1 more source

Focal calcium monitoring with targeted nanosensors at the cytosolic side of endoplasmic reticulum

Science and Technology of Advanced Materials, 2016
Ca2+ distribution is spatially and temporally non-uniform inside cells due to cellular compartmentalization. However, Ca2+ sensing with small organic dyes, such as fura-2 and fluo-4, has been practically applied at a single cell level where the averaged ...
Yanyan Hou, Satoshi Arai, Yoshiaki Takei, Atsushi Murata, Shinji Takeoka, Madoka Suzuki   +5 more
doaj   +1 more source

Alternative method to detect compounds produced by Gambierdiscus spp.

Frontiers in Marine Science, 2014
Ciguatoxins (CTXs) and CTX precursors are produced by several Gambierdiscus spp. These polyether toxins are associated to ciguatera fish poisoning (CFP). In addition to CTX, maitotoxins (MTX) and gambierol are also produced by these dinoflagellates.
Jon Andoni Sánchez
doaj   +1 more source