Results 21 to 30 of about 24,447,932 (192)

A Novel Lensless Miniature Contact Imaging System for Monitoring Calcium Changes in Live Neurons

open access: yesIEEE Photonics Journal, 2014
Here, we report on the design, fabrication, and verification of a novel CMOS-imager-based contact imaging system. We acquired fluorescent images from live neurons by monitoring calcium changes with Fura-2 dye.
Anil Kumar Mudraboyina   +4 more
doaj   +1 more source

Caulerpenyne Affects Bradykinin-Induced Intracellular Calcium Kinetics in LoVo Cells

open access: yesApplied Sciences, 2021
Sesquiterpene caulerpenyne (CYN) is the major metabolite present in green macroalgae Caulerpa taxifolia. This metabolite has been shown to be cytotoxic in some cell lines and was found to be active in various assays of pharmacological interest.
Giovanna Traina
doaj   +1 more source

The air-breathing Alaska blackfish (Dallia pectoralis) remodels ventricular Ca2+ cycling with chronic hypoxic submergence to maintain ventricular contractility

open access: yesCurrent Research in Physiology, 2022
The Alaska blackfish (Dallia pectoralis) is a facultative air-breather endemic to northern latitudes where it remains active in winter under ice cover in cold hypoxic waters.
Holly A. Shiels   +6 more
doaj   +1 more source

Calcium Imaging of Cortical Neurons using Fura-2 AM [PDF]

open access: yesJournal of Visualized Experiments, 2009
Calcium imaging is a common technique that is useful for measuring calcium signals in cultured cells. Calcium imaging techniques take advantage of calcium indicator dyes, which are BAPTA-based organic molecules that change their spectral properties in response to the binding of Ca2+ ions.
Barreto-Chang, Odmara L   +1 more
openaire   +2 more sources

Cytotoxicity-related effects of imidazolium and chlorinated bispyridinium oximes in SH-SY5Y cells

open access: yesArhiv za Higijenu Rada i Toksikologiju, 2022
Current research has shown that several imidazolium and chlorinated bispyridinium oximes are cytotoxic and activate different mechanisms or types of cell death.
Zandona Antonio   +4 more
doaj   +1 more source

Bile Acids Do Not Contribute to the Altered Calcium Homeostasis of Platelets from Rats with Biliary Cirrhosis

open access: yesFrontiers in Physiology, 2017
Previously, we have found that intracellular calcium homeostasis is altered in platelets from an experimental model of liver cirrhosis, the bile-duct ligated (BDL) rat; these alterations are compatible with the existence of a hypercoagulable state and ...
Paola Romecín   +5 more
doaj   +1 more source

Altered calcium signaling in platelets from nitric oxide-deficient hypertensive rats

open access: yesCell Communication and Signaling, 2004
Background In the present study we have analyzed the mechanisms of calcium entry and mobilization in platelets obtained from rats chronically treated with the nitric oxide synthesis inhibitor, N-nitro L-arginine methyl ester [L-NAME, 40 mg/kg/day, 5 days)
Ortiz M Clara   +4 more
doaj   +1 more source

Excitation-contraction coupling in intact frog skeletal muscle fibers injected with mmolar concentrations of fura-2 [PDF]

open access: yes, 1992
Experiments were carried out to test the hypothesis that mM concentrations of fura-2, a high-affinity Ca2+ buffer, inhibit the release of Ca2+ from the sarcoplasmic reticulum (SR) of skeletal muscle fibers.
Harkins, A.B.   +4 more
core   +1 more source

Simultaneous monitoring of Zn2+ secretion and intracellular Ca2+ from islets and islet cells by fluorescence microscopy

open access: yesBioTechniques, 2004
A method for simultaneously imaging Zn2+ secretion and intracellular Ca2+ at β-cell clusters and single islets of Langerhans was developed. Cells were loaded with the Ca2+ indicator Fura Red, incubated in buffer containing the Zn2+ indicator FluoZin-3 ...
Wei-Jun Qian   +4 more
doaj   +1 more source

Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles

open access: yesStress Biology, 2022
The plant vacuole plays a fundamental role in cell homeostasis. The successful application of patch-clamp technique on isolated vacuoles allows the determination of the functional characteristics of tonoplast ion channels and transporters.
Antonella Gradogna, Armando Carpaneto
doaj   +1 more source

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