Results 81 to 90 of about 45,846 (301)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Adsorption and release of BMP-2 on nanocrystalline apatite-coated and uncoated hydroxyapatite/b-tricalcium phosphate porous ceramics [PDF]

, 2009
The association of bone morphogenetic proteins (BMPs) with calcium phosphate bioceramics is known to confer them osteoinductive properties. The aim of this study was to evaluate the surface properties, especially regarding recombinant human BMP-2 (rhBMP-
Alam, Baig, Barroug, Barroug, Benaziz, Bohic, Boix, Charlot, Coradin, Daculsi, Dong, Frolik, Habibovic, Habibovic, Hayakawa, Hughes Wassell, Klenke, Le Nihouannen, Liu, Liu, Matsumoto, Midy, Midy, Murata, Ouizat, Rey, Rey, Rey, Rouahi, Ruppert, Scheufler, Schmoekel, Seeherman, Sumner, Tsujigiwa, Uludag, Vehof, Yuan, Zisch   +38 more
core   +3 more sources

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Hydroxyapatite coating on titanium by a low energy plasma spraying mini-gun [PDF]

, 2012
Plasma-sprayed hydroxyapatite (HA) coatings are used on metallic implants to improve osseointegration and bone growth. The purpose of this work was to determine the microstructure and composition of HA coatings obtained with a newly developed low energy ...
Barykin, George, Braceras, Inigo, Combes, Christèle, Demnati, Imane, Drouet, Christophe, Fagoaga, Ignacio, Gonçalvès, Stéphane, Grossin, David, Parco, Maria, Rey, Christian   +9 more
core   +2 more sources

Porous calcium phosphate ceramics prepared by coating polyurethane foams with calcium phosphate cements [PDF]

Materials Letters, 2004
Porous calcium phosphates have important biomedical applications such as bone defect fillers, tissue engineering scaffolds and drug delivery systems. While a number of methods to produce the porous calcium phosphate ceramics have been reported, this study aimed to develop a new fabrication method.
Miao, Simon, Hu, Yifei, Liu, Jianli, Wong, A   +3 more
openaire   +2 more sources

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Study of adhesion of composite coatings to the implant depending on the method of surface preparation [PDF]

, 2017
In the study the coatings were obtained on titanium substrates using four different methods: etching of substrates in an aqueous solution of nitric and hydrofluoric acids; forming of the oxide layer on the surface using electrochemical oxidation of the ...
Больбасов, Евгений Николаевич, Мещерякова, М. А., Харапудченко, Е. А.   +2 more
core  

Influence of Nanoparticles Deposition Conditions on the Microarc Coatings Properties [PDF]

, 2017
The surface charge of biomaterials significantly contributes to such processes as protein adsorption or biofilm formation and consequently osseointegration bone tissue and implant.
Chebodaeva, Valentina Vadimovna, Sedelnikova, M., Sharkeev, Yury Petrovich   +2 more
core   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Modification of the zirconia ceramics by different calcium phosphate coatings:comparative study

, 2017
The aim of this study was to characterize different calcium phosphate coatings and evaluate in vitro cell response of these materials to ceramics implants.
Ashrafov, A. H, Bolbasov, E. N., Golovkin, A. S., Kozelskaya, A. I., Mishanin, A. I., Novikov, V. A., Shesterikov, E. V., Tverdokhlebov, S. I., Viknianshchukb, A. N.   +8 more
core