Results 61 to 70 of about 1,131,166 (283)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Bioactive calcium phosphate coatings on metallic implants

AIP Conference Proceedings, 2017
Biocomposites based on bioinert metals or alloys and bioactive calcium phosphate coatings are a promising tendency of the new-generation implants development. In recent years, the approach of regenerative medicine based on the use of biodegradable biomaterials has been priority direction.
Sedelnikova, M. B., Komarova, E. G., Sharkeev, Yury Petrovich, Tolkacheva, T. V., Khlusov, Igor Albertovich, Sheikin, V. V.   +5 more
openaire   +2 more sources

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Dialysis Preparation of Smart Redox and Acidity Dual Responsive Tea Polyphenol Functionalized Calcium Phosphate Nanospheres as Anticancer Drug Carriers

Molecules, 2020
Large-scale preparation of biocompatible drug delivery systems with targeted recognition and controlled release properties has always been attractive. However, this strategy has been constrained by a lot of design challenges, such as complicated steps ...
Xiuli Ren, Peng Zhang, Zhenhua Chen
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Preliminary characterization of calcium chemical environment in apatitic and non-apatitic calcium phosphates of biological interest by X-ray absorption spectroscopy [PDF]

, 2005
Several reports have mentioned the existence of non-apatitic environments of phosphate and carbonate ions in synthetic and biological poorly crystalline apatites.
Susini, Jean, Eichert, Diane, Salomé, Murielle, Banu, Mihai, Rey, Christian   +4 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Comparative characteristics of osseointegration processes of the calcium phosphate coating implants and implants with germanium enriched calcium phosphate coatings

N.N. Priorov Journal of Traumatology and Orthopedics, 2017
Introduction. Osseointegration process exerts considerable influence on the term of biological fixation of the implants and is an important parameter that is to be focused on at the implant placement. Purpose: to conduct a comparative analysis of the osseointegration of the titanium alloy implants with different coatings for the determination of the ...
E. A. Zelichenko, V. V. Guzeev, Ya. B. Koval’skaya, O. A. Gurova, T. I. Guzeeva   +4 more
openaire   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Antibacterial Calcium Phosphate Coatings with Zinc Oxide Nanoparticles

, 2023
Porous calcium phosphate coatings (C-P) with ZnO nanoparticles were obtained via the micro-arc oxidation method on a titanium substrate. ZnO nanoparticles were added to the C-P coatings to change the zeta potential and improve the coatings&rsquo ...
Olga Bakina, Yurii Sharkeev, Andrey Miller, Aleksander Zakharenko, Margarita Khimich, Vladimir Egorkin, Mariya Sedelnikova, Igor Vyaliy, Valentina Chebodaeva, Alexey Tolmachev, Kirill Golohvast   +10 more
core   +1 more source