Results 61 to 70 of about 1,164,355 (261)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Results from Geometric and Radiometric Calibration of TerraSAR-X [PDF]

, 2007
As TerraSAR-X, due for launch in 2007, will be an operational scientific mission with commercial potential, product quality is of crucial importance. The success or failure of the mission essentially depends on the calibration of the TerraSAR-X system ...
Döring, Björn, Schwerdt, Marco, Bachmann, Markus, Bräutigam, Benjamin   +3 more
core  

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Metrological characterization of standards – plastic films, which are used for calibration of nano volume spectrophotometers [PDF]

EPJ Web of Conferences
UV-VIS spectrophotometers, including nano drop (volume) spectrophotometers, provide an answer to all challenges in scientific and industrial laboratories, whether for pharmaceutical, chemical or service laboratories, all the way to more complex ...
Zarubica Veljko, Kubičela Edvard, Zarkov Boban, Srećković Milesa   +3 more
doaj   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

FEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno, Shota Yamazaki, Hiromichi Hoshina, Masahiko Harata   +3 more
wiley   +1 more source

A simplified algorithm for leaky network analyzer calibration [PDF]

, 1995
A new algorithm for network analyzer calibration is presented. The error model includes leakage effects and can be applied to a general n-port NWA. The 2-port 16-term model becomes a special case of this new technique which is also hopefully suitable for
F. Sanpietro, Sanpietro, F., Ferrero, Andrea Pierenrico   +2 more
core   +1 more source

Research And Experimental Verification of Absolute Measurement Facility Parameters for Radionuclide Neutron Source Emission Rate

Journal of Isotopes
The manganese bath method is a commonly used method for determining neutron source emission rate.The Monte Carlo method (MCNP) was used to calculate the influence of main parameters such as bath radius，MnSO4 solution density，and measurement chamber ...
Haixian ZHANG, Yina LIU, Zhiqiang WANG, Chunjuan LI, Mingzhe SONG, Hailong LUO   +5 more
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source