Results 151 to 160 of about 39,056 (282)

Establishment of a cryopreservation process for long-term storage of embryogenic calli of Hevea brasiliensis (Müll. Arg.). [PDF]

, 2006
A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The technique included a callus pre-culture on a maintenance medium containing 1 mM CaCl2 prior to cryopreservation to promote postthaw callus
Carron, Marc-Philippe, Chapuset, Thierry, Lardet, Ludovic, Martin, Florence, Montoro, Pascal   +4 more
core  

Annual Research Review: Early conduct problems – precursors, outcomes, and etiology

Journal of Child Psychology and Psychiatry, EarlyView.
During the toddler and preschool period, nearly all children engage in some level of aggression, defiance, stealing, and temper tantrums. While the frequency and intensity of these behaviors tends to decrease across early childhood, a subset of children engage in these conduct problem behaviors at a higher intensity early in life and/or do not desist ...
Luke W. Hyde, Christopher J. Trentacosta, Jessica L. Bezek   +2 more
wiley   +1 more source

Enhancing tomato regeneration and genetic transformation efficiencies via exogenous chemical treatment and the GRF5 gene modulation

Journal of Integrative Plant Biology, EarlyView.
Supplementation of Driver and Kuniyuki Walnut Medium with phloroglucinol enhanced regeneration efficiency in tomato tissue culture. Heterologous expression of an Arabidopsis growth‐regulating factor gene, GROWTH‐REGULATING FACTOR5 (GRF5), in tomato improved regeneration and transformation efficiency, suggesting a synergistic effect between ...
Junjie Rong, Lei Zhu, Qingfeng Niu, Jianru Zhao, Fengjuan Chu, Jian‐Kang Zhu, Zhaobo Lang   +6 more
wiley   +1 more source

OsSDG715, a Histone H3K9 Methyltransferase, Integrates Auxin and Cytokinin Signaling to Regulate Callus Formation in Rice

Rice
Efficient callus induction is essential for the genetic transformation of rice (Oryza sativa), yet its regulatory mechanisms remain elusive. Previously, through a genome-wide association study (GWAS), we identified a significant associated locus on ...
Wenjing Song, Hairong Cai, Yuanyuan Guo, Shiyi Chen, Yingyun Yao, Jiafeng Wang, Tao Guo, Jian Zhang, Chun Chen   +8 more
doaj   +1 more source

Evaluating Tartary Buckwheat Genotypes with High Callus Induction Rates and the Transcriptomic Profiling during Callus Formation. [PDF]

Plants (Basel), 2023
Zhao H, Li X, Xiao X, Wang T, Liu L, Li C, Wu H, Shan Z, Wu Q.   +8 more
europepmc   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Machine Learning Analysis of the Impact of Silver Nitrate and Silver Nanoparticles on Wheat (Triticum aestivum L.): Callus Induction, Plant Regeneration, and DNA Methylation. [PDF]

Plants (Basel), 2023
Türkoğlu A, Haliloğlu K, Demirel F, Aydin M, Çiçek S, Yiğider E, Demirel S, Piekutowska M, Szulc P, Niedbała G.   +9 more
europepmc   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, EarlyView.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Cloning crops in a CELSS via tissue culture: Prospects and problems [PDF]

Micropropagation is currently used to clone fruits, nuts, and vegetables and involves controlling the outgrowth in vitro of basal, axillary, or adventitious buds. Following clonal multiplication, shoots are divided and rooted.
Carman, John G., Hess, J. Richard
core   +1 more source

Optimizations of Cas12a‐ and Cas12i‐based adenine base editors for efficient precision editing in the plant genome

Journal of Integrative Plant Biology, EarlyView.
A strategy coupling high‐activity nucleases with dimeric TadA‐8e optimizes plant Cas12‐based adenine base editors, boosts editing efficiency, and provides precise editors for crop breeding and genomics research.
Xiaoshuang Liu, Shan Jin, Zhi Xiao, Chong Ma, Qing Wang, Huanhuan Wang, Ruohan Zhou, Dongfang Gu, Rongfang Xu, Ruiying Qin, Juan Li, Pengcheng Wei   +11 more
wiley   +1 more source