Results 71 to 80 of about 171,183 (310)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Capacitance-Enhanced Microsupercapacitors with a Redox Electrolyte Additive for an All-MXene-Based Flexible Self-Powered Integrated Photodetecting System

open access: yes, 2023
Although much effort has been devoted to improving the electrochemical performances of MXene-based metal-ion hybrid microsupercapacitors (MSCs), few works focus on the electrolyte.
Guozhen Shen (1403410)   +7 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Radiation effects in polycarbonate capacitors [PDF]

open access: yesNuclear Technology and Radiation Protection, 2009
The aim of this paper is to examine the influence of neutron and gamma irradiation on the dissipation factor and capacitance of capacitors with polycarbonate dielectrics.
Vujisić Miloš   +3 more
doaj   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

open access: yesFEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura   +19 more
wiley   +1 more source

Capacitance-voltage Profiling Techniques for Characterization of Semiconductor Materials and Devices

open access: yes, 2018
A new capacitance-voltage profiling technique of semiconductor junctions is proposed for characterisation of semiconductor materials and devices. The measurement technique is simple, non-destructive and it has a greater accuracy compared with the ...
Miron J. Cristea (5008901)
core   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

THE EFFECT OF FREQUENCY VARIATION ON DIELECTRIC CONSTANT OF RAPHIA HOOKERI

open access: yesJournal of Renewable Energy and Mechanics, 2020
This work sought to investigate the effect of varying the frequency of an applied electric field on the dielectric constant of raphia hookeri. The aim of the work was to ascertain the suitability of using the sample for engineering applications.
Ubong Iboh   +3 more
doaj   +1 more source

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