Results 111 to 120 of about 583,418 (306)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Student-Led IEPs: How to Make it Work (NCSET Capacity Building Institute)

open access: yes, 2002
A transcript of a Capacity Building Institute (CBI) that was co-sponsored by NCSET and PACER Center. It provided an opportunity to present information from some of the key authors and practitioners of Student-Led IEPs.
National Center on Secondary Education and Transition
core  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Creating Youth Development Systems that Include Youth with Disabilities (NCSET Capacity Building Institute)

open access: yes, 2001
A transcript of a Capacity Building Institute (CBI) that was an opportunity for key community stakeholders in education, workforce development, and disability services to examine how to better align the various youth development investment systems so ...
National Center on Secondary Education and Transition
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Capacity building towards sustainability: Context of post disaster waste management

open access: yes
Concept of Capacity Building is an essential component in development theory and practice. In developing countries, it is identified as a key concept in achieving sustainability. In particular, in post disaster scenarios, focus has been placed upon local
Amaratunga, Dilanthi   +2 more
core  

A Transition Odyssey Technical Assistance about Transition and the Rehabilitation Act (NCSET Capacity Building Institute)

open access: yes, 2001
A transcript of a Capacity Building Institute (CBI) that was sponsored by NCSET and PACER Center. It included nationally recognized speakers on transition planning and discussion groups focusing on parent involvement in postsecondary education ...
National Center on Secondary Education and Transition
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Preparing Youth with Disabilities for Successful Participation in Postsecondary Education and Employment (NCSET Capacity Building Institute)

open access: yes, 2001
A transcript of a Capacity Building Institute (CBI) that was sponsored by NCSET and the National Center for the Study of Postsecondary Educational Supports.
National Center on Secondary Education and Transition
core  

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

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