Results 101 to 110 of about 228,053 (289)

Bioorthogonal Fluorogenic Reporters for Noninvasive Imaging and Urinalysis of Immunotherapeutic Response in Renal Cell Carcinoma

Advanced Science, EarlyView.
This study reported renal‐clearable bio‐orthogonal near‐infrared fluorogenic probes (BGRs) that specifically imaging and urinalysis of granzyme B for dynamic evaluation of RCC immunotherapy. BGRs not only differentiate immunotherapeutic responses in orthotopic RCC mice, but also enable sensitive optical urinalysis of granzyme B in clinical specimens ...
Xingyue Yang, Baoshuai Liang, Liangmin Fu, Weiliang Deng, Yuyan Jiang, Weiping Xu, Jinwei Chen, Ya Zhou, Ke Wu, Jiaguo Huang   +9 more
wiley   +1 more source

New analytical challenges in characterization of antibody-drug conjugates

Journal of Chromatography Open
Since 1913, when Paul Ehrlich introduced the concept of “selective toxicity”, new strategies to obtain quality control, quantification of the drug were mandatory. For this reason, the introduction of antibody drug conjugates (ADC) has shown many critical
Erika Maria Ricci, Enrico Dainese, Vincenzo De Laurenzi, Giulio Lovato, Gianluca Sala, Marcello Locatelli, Miryam Perrucci   +6 more
doaj   +1 more source

Plant‐Derived Melatonin Inhibits Bacterial Virulence via CpxA/R Two‐Component System

Advanced Science, EarlyView.
Plant‐derived melatonin is sensed by CpxAE48/T51, which inhibits the phosphorylation cascade transmission from CpxAH240 to CpxRD52, resulting in the inhibition of DNA‐binding capacity of CpxR and subsequent T3SS genes expression in Pst DC3000. ABSTRACT In defending against pathogens, plants deploy diverse secondary metabolites and signaling molecules ...
Jin‐Wei Wei, Wei Liu, Bili Cao, Dan Zhao, Chengqiang Wang, Xin Hou, Tangyuan Ning, Biao Gong   +7 more
wiley   +1 more source

Structurally Enhanced Colloidal Molecules via Carbene‐Mediated Covalent C─H Insertion Crosslinking

Advanced Science, EarlyView.
This work presents a nonspecific covalent crosslinking strategy to significantly enhance the structural stability of colloidal molecules (CMs) without compromising their inherent structural and physicochemical properties by rationally designing a diazo‐based crosslinker.
Liwei Dai, Chongyang Yao, Xiancheng Zhang, Weijia Kong, Mingxing Liu, Huibin He, Yutao Sang, Zhihong Nie   +7 more
wiley   +1 more source

Possibilities of use of method of capillary electorphoresis while analyzing aqueous extract from saline soils

Бюллетень Почвенного института им. В.В. Докучаева, 2016
A comparative analysis of anions (Cl-, SO4 2-) and cations (Ca2+, Mg2+, Na+) detection in samples of saline soils by classical methods of titration (with use of atomic absorption analyzer Kvant-2AT for Na+ detection) and by method of capillary ...
G. I. Chernousenko, T. I. Borisochkina, N. S. Nikitina   +2 more
doaj   +1 more source

Optical sensing in microchip capillary electrophoresis by femtosecond laser written waveguides [PDF]

, 2009
Capillary electrophoresis separation in an on-chip integrated microfluidic channel is typically monitored with bulky, bench-top optical excitation/detection instrumentation.
Cerullo, G., Chiari, M., Cretich, M., Dongre, C., Hoekstra, H.J.W.M., Martinez-Vázquez, R., Osellame, R., Pollnau, M., Ramponi, R., Vlekkert, H. van den   +9 more
core   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Advances in Microfluidics and Lab-on-a-Chip Technologies

, 2017
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
Abate, Adley, Aebersold, Ahlford, Almassian, Almoguera, Asiello, Athamanolap, Baker, Becker, Blazej, Brehm-Stecher, Burger, Business-Wire, Byrnes, Cai, Cao, Chang, Chao, Chatterjee, Chin, Chin, Coupland, Craw, Crews, Crews, Czilwik, Derveaux, DeVoe, Dharmasiri, Dodsworth, Domon, Duffy, Erlich, Farrar, Fredrickson, Fu, Gagnon, Gao, Gonzalez de Castro, Grover, Hadd, Han, Hanyoup, Hardouin, Hauck, Hayden, He, He, He, Hindson, Ho, Hsieh, Huang, Hughes, Jacobs, Jansson, Jarvius, Jayamohan, Jayamohan, Jenkins, Jha, Ji, Jin, Jin, Kalra, Karimi, Karns, Khandurina, Kim, Kim, Kim, Kim, Kim, Klein, Klostranec, Konry, Kuroda, Kwasny, Kwasny, Kühnemund, Küster, Lagally, Lapierre, Lazar, Lazar, Lee, Lee, Lee, Li, Liu, Lochovsky, Macosko, Mahmoudian, Mani, Manz, Mao, Mariella, Melin, Mery, Metzker, Mezger, Mitchell, Mitchell, Neuzil, Ng, Northen, Oblath, Oxford Nanopore, Paegel, Paegel, Paliwal, Park, Pekin, Pennisi, Perez-Toralla, Pješčić, Pješčić, Pješčić, Prakash, Priye, Priye, Quick, Ro, Romanov, Safavieh, Sainiemi, Samuel, Sanvicens, Sato, Sayad, Schoenitz, Schuler, Shamsi, Shih, Song, Srbek, Stumpf, Sun, Sundberg, Tanaka, Teh, Tehranirokh, Thompson, Thuy, Tian, Tröger, Unger, Vergauwe, Volpatti, Weisenberger, Wheeler, Whitesides, Woolley, Xie, Xu, Xue, Yetisen, Yin, Zhang, Zhang, Zhu   +161 more
core   +1 more source

Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform

Advanced Science, EarlyView.
We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng, Jiahao Lin, Qiushuang Zhang, Xiangdong Zhang, Yicong Dai, Jinghong Li   +5 more
wiley   +1 more source

DNA Nanoarray for Multiplexed RNA Detection With Single‐Molecule Readout

Advanced Science, EarlyView.
A carrier‐based DNA nanoarray integrates programmable nucleic acid hybridization with solid‐state nanopore readout for direct RNA detection at single‐molecule resolution. A ternary encoding system enables 27 carriers with 81 addressable sensing sites, allowing the simultaneous identification of targets spanning a wide size range, from short microRNAs ...
Yunxuan Li, Yesheng Wang, Chunmiao Yu, Siyu Hou, Elli Mylona, Plamena Naydenova, Jingyuan Zhao, Hong Yuan, Jingsong Duan, Hendrik F. P. Runge, Stephen Baker, Jinbo Zhu, Ulrich F. Keyser   +12 more
wiley   +1 more source